Peptide-Modified "Smart" Hydrogels and Genetically Engineered Stem Cells for Skeletal Tissue Engineering
Peptide-Modified "Smart" Hydrogels and Genetically Engineered Stem Cells for Skeletal Tissue Engineering
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DOI：
10.1021/bm100157s
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发表时间：
2010-06-01
影响因子：
6.2
通讯作者：
Gazit, Dan
中科院分区：
文献类型：
--
作者：
Garty, Shai;Kimelman-Bleich, Nadav;Gazit, Dan
刺激响应或“智能”水凝胶对于组织工程应用是感兴趣的，其具有微创应用的优点。目前，这些材料尚未被用作恢复受损组织或器官功能的生物替代品。本研究的目的是证明温敏性含肽水凝胶作为基因工程干细胞的支持基质的优势。我们使用可注射的水凝胶，使细胞输送到所需的网站，并提供足够的支架植入后。基于聚氧化乙烯和聚氧化丙烯的两亲性嵌段共聚物，用甲基丙烯酸酯或马来酰亚胺实体封端，并进一步与含RGD的肽反应，开发了温度响应性水凝胶。研究了这些水凝胶内的细胞代谢活性和存活，说明稳定的肽聚合物缀合物是延长细胞支持所必需的。独特的聚合物特性，结合其增强的细胞相互作用，表明这些生物材料在各种组织工程应用中的潜在用途。
Stimuli responsive or "smart" hydrogels are of interest for tissue-engineering applications, featuring the advantages of minimally invasive application. Currently, these materials have yet to be used as a biological replacement in restoring the function of damaged tissues or organs. The aim of this study was to demonstrate the advantages of thermoresponsive, peptide-containing hydrogels as a supportive matrix for genetically engineered stem cells. We used injectable hydrogels, enabling cell delivery to the desired site and providing adequate scaffolding postimplantation. Thermoresponsive hydrogels were developed based on amphiphilic block copolymers of polyethylene-oxide and polypropylene-oxide end-capped with methacrylate or maleimide entities and further reacted with RGD-containing peptides. Cell metabolic activity and survival within those hydrogels was studied, illustrating that the stable peptide polymer conjugate is required for prolonged cell support. The unique polymer characteristics, combined with its enhanced cell interactions, suggest the potential use of these biomaterials in various tissue engineering applications.