Memory formation requires p38MAPK activity in the rat hippocampus
Memory formation requires p38MAPK activity in the rat hippocampus
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DOI：
10.1097/00001756-200310270-00022
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发表时间：
2003-10-27
期刊：
影响因子：
1.7
通讯作者：
Cammarota, M
中科院分区：
文献类型：
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作者：
Alonso, M;Bevilaqua, LRM;Cammarota, M
使用p38MAPK的特异性抑制剂SB203580，我们发现该蛋白激酶直接参与短期和长期记忆。在一次试验的抑制性回避任务训练后立即给予大鼠海马背侧CAI区域，而不是在30或120分钟后给予，SB203580阻断了短期和长期记忆的形成。SB203580无效模拟SB202474没有任何影响。回避任务的学习伴随着海马p38MAPK磷酸化的立即和短暂的增加。在只接受与学习范式相关的足电刺激的对照动物中，未检测到p38MAPK磷酸化的变化。因此，抑制回避的短期和长期记忆的形成需要大鼠海马中p38MAPK的激活。
Using the specific inhibitor of p38MAPK, SB203580, we show a direct involvement of this protein kinase in short- and long-term memory. When given into the CAI region of the rat dorsal hippocampus immediately, but not 30 or 120 min after training in a one-trial inhibitory avoidance task, SB203580 blocked short- and long-term memory formation. The SB203580 inactive analog, SB202474, had no effect whatsoever. Learning of the avoidance task was accompanied by an immediate and transient increase in hippocampal p38MAPK phosphorylation. No change in p38MAPK phosphorylation was detected in control animals that only received the electric foot-shock associated with the learning paradigm. Therefore, formation of short and long-term memory for inhibitory avoidance requires p38MAPK activation in the rat hippocampus.