Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae
Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae
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DOI：
10.1016/j.femsyr.2005.05.004
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发表时间：
2005-12-01
影响因子：
3.2
通讯作者：
Dranginis, AM
中科院分区：
文献类型：
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作者：
Bayly, JC;Douglas, LM;Dranginis, AM
FLOH编码的絮凝蛋白是酿酒酵母中多种重要表型所必需的，包括絮凝、对琼脂和塑料的粘附、侵入性生长、假菌丝形成和生物膜发育。我们目前的证据表明，Flo 11 p属于Flo 1型类的絮凝，而不是NewFlo类。Flo 1型和NewFlo型酵母的絮凝作用均受到甘露糖的抑制。然而，NewFlo絮凝也被包括葡萄糖、麦芽糖和蔗糖在内的几种其他碳水化合物抑制。在至少一种情况下，这些差异已被证明反映了絮凝蛋白的碳水化合物结合位点的结构差异。我们报告说，Flo 11 p依赖的絮凝抑制甘露糖，但不是由葡萄糖，麦芽糖或蔗糖。此外，Flo 11 p含有一个与已被证明能抑制Flol型絮凝蛋白的肽序列高度相似的肽序列。Flo 11 p依赖性絮凝特性的进一步表征表明，它依赖于钙，仅在细胞密度大于1 × 10（8）ml（-1）时发生，并且仅在酸性pH值下发生。（c）2005年欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
The FLOH-encoded flocculin is required for a variety of important phenotypes in Saccharomyces cerevisiae, including flocculation, adhesion to agar and plastic, invasive growth, pseudohyphae formation and biofilm development. We present evidence that Flo11p belongs to the Flo1-type class of flocculins rather than to the NewFlo class. Both Flo1-type and NewFlo yeast flocculation are inhibited by mannose. NewFlo flocculation, however, is also inhibited by several other carbohydrates including glucose, maltose and sucrose. These differences have in at least one case been shown to reflect differences in the structure of the carbohydrate-binding site of the flocculins. We report that Flo11p-dependent flocculation is inhibited by mannose, but not by glucose, maltose or sucrose. Furthermore, Flo11p contains a peptide sequence highly similar to one that has been shown to characterise Flol-type flocculins. Further characterisation of the properties of Flo11p-dependent flocculation revealed that it is dependent on calcium, occurs only at cell densities greater than 1 x 10(8) ml(-1), and only occurs at acidic pH. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.