Simultaneous photoacoustic imaging of intravascular and tissue oxygenation
Simultaneous photoacoustic imaging of intravascular and tissue oxygenation
复制标题
血管内和组织氧合的同步光声成像
DOI：
10.1364/ol.44.003773
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发表时间：
2019-08-01
期刊：
影响因子：
3.6
通讯作者：
Yao, Junjie
中科院分区：
文献类型：
--
作者：
Chen, Maomao;Knox, Hailey J.;Yao, Junjie
缺氧是由氧气供应不足引起的一种低组织氧合状态，会导致潜在的不可逆转的组织损伤，如中风期间的脑梗塞。长期以来，血管内氧合一直被光声成像和其他成像手段用于研究缺氧。然而，血管内氧合仅描述了通过微循环的氧气供应，而不能直接反映间质液体中可用于新陈代谢的游离氧量。因此，为了充分了解缺氧，在同一生物过程中监测血液氧合和组织氧合是非常必要的。在这项工作中，通过结合高分辨率光声显微镜(PAM)和一种新型的基于N-氧化物的生物还原缺氧敏感探针Hyp-650，我们展示了血管内氧合和组织缺氧的同时成像。我们已经确定了Hyp-650在体外和活细胞中用于低氧激活的详细的化学、光学和光声性质。我们还在后肢缺血模型和荷瘤小鼠上进行了PAM，以研究不同低氧水平下血管内氧合和组织氧合的相关性。我们预计Hyp-650增强光声成像将在脑和癌症研究中找到各种应用。(C)2019年美国光学学会
Hypoxia, a low tissue oxygenation condition caused by insufficient oxygen supply, leads to potentially irreversible tissue damage, such as brain infarction during stroke. Intravascular oxygenation has long been used by photoacoustic imaging, among other imaging modalities, to study hypoxia. However, intravascular oxygenation describes only the oxygen supply via microcirculation, which does not directly reflect the amount of free oxygen available for metabolism in the interstitial fluid. Therefore, to fully understand hypoxia, it is highly desirable to monitor blood oxygenation as well as tissue oxygenation during the same biological process. In this work, by combining high-resolution photoacoustic microscopy (PAM) and a novel bioreducible N-oxide-based hypoxia-sensitive probe HyP-650, we have demonstrated simultaneous imaging of intravascular oxygenation and tissue hypoxia. We have established detailed chemical, optical, and photoacoustic properties of HyP-650 for hypoxic activation in vitro and in living cells. We have also performed PAM on hindlimb ischemia models and tumor-bearing mice to study the correlation between intravascular oxygenation and tissue oxygenation at various hypoxic levels. We expect that Hyp-650 enhanced photoacoustic imaging will find a variety of applications in brain and cancer research. (c) 2019 Optical Society of America