Equilibrium separation and filtration of particles using differential inertial focusing
Equilibrium separation and filtration of particles using differential inertial focusing
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DOI：
10.1021/ac702283m
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发表时间：
2008-03-15
影响因子：
7.4
通讯作者：
Toner, Mehmet
中科院分区：
文献类型：
--
作者：
Di Carlo, Dino;Edd, Jon F.;Toner, Mehmet
溶液中颗粒的快速分离和过滤具有广泛的应用，包括血细胞分离、超声造影剂制备和发酵产物纯化。然而，提供快速处理速率的当前技术复杂度高。我们提出了一种快速的微流体过滤技术，能够根据大小分离颗粒，纯度从90至100%和高容量的1毫升/分钟的吞吐量。数据分离刚性颗粒，可变形的乳液，和血小板从全血。该系统是基于不同尺寸的颗粒的差分惯性聚焦，并且允许仅基于在通过不对称弯曲通道的流动中产生的固有流体动力的连续分离。开发的基础力量的理论描述，并结合数据确定的大小切断分离，半经验关系描述如何通道的几何形状与此切断显示。串联的级联分离显示出对于提高纯度和产率是有用的。这种类型的微流体系统可以过滤可变形颗粒，在很大程度上不依赖于颗粒密度，并且可以以紧凑的形式提供宏观尺度过滤的典型吞吐量，使得能够应用于血液过滤和颗粒浓缩。
Rapid separation and filtration of particles in solution has a wide range of applications including blood cell separation, ultrasound contrast agent preparation, and purification of fermentation products. However, current techniques that provide quick processing rates are high in complexity. We present a rapid microfluidic filtration technology capable of separating particles based on size, with purities from 90 to 100% and high-volume throughputs of 1 mL/min. Data for separation of rigid particles, deformable emulsions, and platelets from whole blood are presented. The system is based upon differential inertial focusing of particles of varying sizes and allows continuous separation based only on intrinsic hydrodynamic forces developed in a flow through an asymmetrically curved channel. A theoretical description of the underlying forces is developed, and in combination with data determining a size cutoff for separation, a semiempirical relationship describing how channel geometry is related to this cutoff is shown. Cascading separations in series is shown to be useful for increasing purity and yield. This type of microfluidic system can filter deformable particles, is largely independent of particle density, and can provide throughputs typical of macroscale filtration in a compact format, enabling applications in blood filtration and particle concentration.