Eph-ephrin A system regulates murine blastocyst attachment and spreading
Eph-ephrin A system regulates murine blastocyst attachment and spreading
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DOI：
10.1002/dvdy.20977
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发表时间：
2006-12-01
影响因子：
2.5
通讯作者：
Fujii, Shingo
中科院分区：
文献类型：
--
作者：
Fujii, Haruko;Tatsumi, Keiji;Fujii, Shingo
虽然哺乳动物子宫内膜上皮细胞表达大量的黏附分子，但目前还没有任何关于防止胚胎过早附着的机制的研究。在这项研究中，我们研究了Eph-eparin相互作用的可能参与，该相互作用可以诱导排斥力。在小鼠中，EphA1、A2和A4在子宫内膜上皮细胞上表达，Ephin A1-4在胚泡上表达。逆转录-聚合酶链式反应显示胚胎中ePhin A1-4的mRNA表达在着床期前后一过性下降。免疫组织化学显示EphA1在子宫内膜上皮细胞上的表达，在胚胎上的表达在着床点下降。重组EphA1与滋养外胚层细胞表面的细胞发生反应。使用涂有EphA1的培养皿进行的附着分析表明，EphA1可逆地抑制胚泡附着。这些发现表明，在胚胎植入前的串扰期间，Eph-eparin A系统在调节最初的胚胎-母体接触方面发挥了重要作用。
Although numerous adhesion molecules are expressed on mammalian endometrial epithelial cells, there have not been any studies of a mechanism to prevent premature attachment of the embryo. In this study, we examined the possible involvement of Eph-ephrin interaction, which can induce repulsive forces. In mice, Eph A1, A2, and A4 were expressed on endometrial epithelial cells and ephrin A1-4 on blastocysts. Reverse transcriptase-polymerase chain reaction showed that mRNA expression of ephrin A1-4 on embryos transiently decreased around the implantation period. Immunohistochemistry demonstrated that the expression of Eph A1 on endometrial epithelial cells and ephrin A1 and A3 expression on embryos decreased at implantation sites. Recombinant Eph A1 reacted with cell the surface of ephrin A-bearing trophectoderm cells. Attachment assays using Eph A1-coated dishes showed that blastocyst attachment was reversibly inhibited by Eph A1. These findings suggest an important role of the Eph-ephrin A system in regulating the initial embryo-maternal contact during the cross-talk period that precedes embryo implantation.