X-ray micro-computed tomography (μCT): an emerging opportunity in parasite imaging.
X-ray micro-computed tomography (μCT): an emerging opportunity in parasite imaging.
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DOI：
10.1017/s0031182017002074
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发表时间：
2018-06
期刊：
影响因子：
2.4
通讯作者：
Withers PJ
中科院分区：
文献类型：
--
作者：
O'Sullivan JDB;Behnsen J;Starborg T;MacDonald AS;Phythian-Adams AT;Else KJ;Cruickshank SM;Withers PJ
X射线微计算机断层扫描（μCT）是一种无需破坏性切片即可获得样品（包括其内部结构）三维图像的技术。在这里，我们审查的能力的技术，并检查其潜力，提供新的见解寄生虫的生活方式嵌入宿主组织。目前的能力和限制的技术在软组织中产生的对比度进行了讨论，以及潜在的解决方案，寄生虫学家希望应用这种技术。我们介绍了小鼠鞭虫Trichuris muris的示例图像，并讨论了μCT的应用，以提供对寄生虫行为和病理学的独特见解，这是其他成像方式无法实现的。
X-ray micro-computed tomography (μCT) is a technique which can obtain three-dimensional images of a sample, including its internal structure, without the need for destructive sectioning. Here, we review the capability of the technique and examine its potential to provide novel insights into the lifestyles of parasites embedded within host tissue. The current capabilities and limitations of the technology in producing contrast in soft tissues are discussed, as well as the potential solutions for parasitologists looking to apply this technique. We present example images of the mouse whipworm Trichuris muris and discuss the application of μCT to provide unique insights into parasite behaviour and pathology, which are inaccessible to other imaging modalities.