INTESTINAL COLONIZATION OF LABORATORY RATS WITH OXALOBACTER-FORMIGENES
INTESTINAL COLONIZATION OF LABORATORY RATS WITH OXALOBACTER-FORMIGENES
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DOI：
10.1128/aem.53.12.2767-2770.1987
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发表时间：
1987-12-01
影响因子：
4.4
通讯作者：
ALLISON, MJ
中科院分区：
文献类型：
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作者：
DANIEL, SL;HARTMAN, PA;ALLISON, MJ
研究人员检查了六种产草酸杆菌（厌氧草酸盐降解细菌）在成年实验大鼠胃肠道中定殖的能力。这些大鼠没有携带 O. formigenes。 OxCR6 菌株是从实验室大鼠的盲肠内容物中分离出来的，该大鼠天然定植有草酸盐降解菌，它也定植于喂食含有 4.5% 草酸钠的饮食的成年大鼠的盲肠和结肠中。大鼠胃内接种109个OxCR6活细胞后5天，盲肠和结肠内容物中的草酸盐降解率分别增加了19倍和40倍。这些大鼠的 OxCR6 菌株活菌数平均为 108/克（干重）盲肠内容物。在饲喂草酸钠含量低于 3.0% 的饮食的接种大鼠的盲肠内容物中未检测到 OxCR6 菌株。从猪、豚鼠和野鼠的盲肠内容物以及人类粪便中分离出的 O. formigenes 菌株也定植于实验室大鼠的盲肠中。瘤胃菌株未能在大鼠盲肠中定殖。
Six strains of Oxalobacter formigenes (anaerobic oxalate-degrading bacteria) were examined for their ability to colonize the gastrointestinal tracts of adult laboratory rats. These rats did not harbor O. formigenes. Strain OxCR6, isolated from the cecal contents of a laboratory rat that was naturally colonized by oxalate-degrading bacteria, colonized the ceca and colons of adult rats fed a diet that contained 4.5% sodium oxalate. Five days after rats were inoculated intragastrically with 109 viable cells of strain OxCR6, oxalate degradation rates in cecal and colonic contents increased by 19 and 40 times, respectively. Viable counts of strain OxCR6 from these rats averaged 108/g (dry weight) of cecal contents. Strain OxCR6 was not detected in the cecal contents of inoculated rats fed diets that contained less than 3.0% sodium oxalate. Strains of O. formigenes isolated from the cecal contents of swine, guinea pigs, and wild rats and from human feces also colonized the ceca of laboratory rats; a ruminal strain failed to colonize the rat cecum.