Increased frequency of circulating follicular helper T cells in patients with rheumatoid arthritis.
Increased frequency of circulating follicular helper T cells in patients with rheumatoid arthritis.
复制标题
类风湿关节炎患者循环滤泡辅助 T 细胞频率增加
DOI：
10.1155/2012/827480
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发表时间：
2012
影响因子：
--
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Ma J;Zhu C;Ma B;Tian J;Baidoo SE;Mao C;Wu W;Chen J;Tong J;Yang M;Jiao Z;Xu H;Lu L;Wang S
滤泡辅助性T（Tfh）细胞被认为是一种独特的CD 4+辅助性T细胞亚群，其提供B细胞活化和产生特异性抗体应答，并且在自身免疫性疾病的发展中起关键作用。到目前为止，只有一项研究调查了SLE患者中循环Tfh细胞的增加。由于对类风湿性关节炎（RA）患者的Tfh细胞知之甚少，在这项研究中，对53例RA患者和31名健康对照者的Tfh细胞频率、相关细胞因子IL-21和转录因子Bcl-6进行了研究。首先，我们发现RA患者外周血中CD 4 + CXCR 5 + ICOSmigh Tfh细胞的频率显著高于健康对照组。已知Tfh细胞对于引导生殖中心B细胞产生抗体应答至关重要;其次，我们观察到Tfh细胞频率伴随着RA患者中抗CCP抗体的水平。RA患者Bcl-6 mRNA表达和血浆IL-21浓度均升高。总之，这些发现表明，循环Tfh细胞的频率增加与抗CCP抗体水平升高相关，表明Tfh细胞可能参与RA的疾病进展。
Follicular helper T (Tfh) cells are recognized as a distinct CD4+ helper T-cell subset, which provides for B-cell activation and production of specific antibody responses, and play a critical role in the development of autoimmune disease. So far, only one study investigated the circulating Tfh cells increased in a subset of SLE patients. Since relatively little is known about the Tfh cells in rheumatoid arthritis (RA) patients, in this study, Tfh-cell frequency, related cytokine IL-21, and transcription factor Bcl-6 were investigated in 53 patients with RA and 31 health controls. Firstly, we found that the frequency of CD4+CXCR5+ICOShigh Tfh cells was increased significantly in the peripheral blood of RA patients, compared with that in healthy controls. It is known that Tfh cells are critical for directing the development of an antibody response by germinal centers B cells; secondly, we observed that the Tfh-cell frequency is accompanied by the level of anti-CCP antibody in RA patients. Furthermore, expression of Bcl-6 mRNA and plasma IL-21 concentrations in RA patients was increased. Taken together, these findings have shown that the increased frequency of circulating Tfh cells is correlated with elevated levels of anti-CCP antibody, indicating the possible involvement of Tfh cells in the disease progression of RA.