n-Alkane uptake and utilisation by Streptomyces strains
n-Alkane uptake and utilisation by Streptomyces strains
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DOI：
10.1023/a:1012030309817
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发表时间：
2001-01-01
影响因子：
2.6
通讯作者：
Szabó, I
中科院分区：
文献类型：
--
作者：
Barabás, G;Vargha, G;Szabó, I
从科威特 Burgan 油田分离的链霉菌菌株被定义为 S. griseoflavus、S. parvus 和 S. plicatus，利用正十六烷、正十八烷（矿物油的纯化馏分）、煤油和原油作为唯一的碳和能源。将菌株与正烷烃一起温育，观察到链长与所用正烷烃相当的脂肪酸含量增加。信号转导 GTP 结合蛋白 (GBP) 在链霉菌的正烷烃摄取中发挥重要作用。 GBP 的特定活化剂增加了碳氢化合物的吸收。使用疏水性荧光染料二苯基六三烯（DPH）作为探针，发现碳氢化合物利用者细胞膜疏水内部区域的微粘度显着低于非利用者。这种差异可能反映了菌株脂肪酸组成的差异。当培养物在含有正烷烃的培养基中生长时，电子显微镜显示碳氢化合物利用者在细胞质中显示出电子密度较低的区域作为内含物。作为对照，接种链霉菌菌株的土壤样品消除碳氢化合物的速度比不含这些菌株的土壤样品快得多。当无机介质以n-hexadecane-1-C-14作为唯一的碳和能源时，检测到放射性CO2。由于链霉菌迄今为止尚未用于消除石油，尽管它们被认为是丰富的耐受极端条件的土壤细菌，因此讨论了它们在碳氢化合物污染土壤的生物修复中的可能用途。
Streptomyces strains isolated from the Kuwait Burgan oil field were defined as S. griseoflavus, S. parvus, and S. plicatus utilised n-hexadecane, n-octadecane (purified fractions of mineral oil), kerosene, and crude oil as sole carbon and energy sources. The strains were incubated with n-alkanes and increase of the fatty acid content with chain length equivalent to the employed n-alkanes was observed. Signal transducing GTP-binding proteins (GBPs) play an important role in n-alkane uptake in streptomycetes. Specific activators of GBPs increased the uptake of hydrocarbons. Using the hydrophobic fluorescent dye diphenylhexatrien (DPH) as a probe, it was found that the microviscosity of the hydrophobic inner region of the cellular membrane is significantly lower in hydrocarbon utilisers than in non-utilisers. This difference probably reflects differences in the fatty acid composition of the strains. When cultures were grown in n-alkane containing media, electron microscopy revealed that the hydrocarbon utilisers showed less-electron dense areas as inclusions in the cytoplasm. Soil samples inoculated with Streptomyces strains eliminated hydrocarbons much faster than those not containing these strains, serving as control. When inorganic medium was supplied with n-hexadecane-1-C-14 as sole carbon and energy source, radioactive CO2 was detected. Since streptomycetes have not been used until now for oil elimination, though they are known as abundant soil bacteria tolerating extreme conditions, their possible use for bioremediation of hydrocarbon contaminated soils is discussed.