HUMAN MACROPHAGE SCAVENGER RECEPTORS - PRIMARY STRUCTURE, EXPRESSION, AND LOCALIZATION IN ATHEROSCLEROTIC LESIONS
HUMAN MACROPHAGE SCAVENGER RECEPTORS - PRIMARY STRUCTURE, EXPRESSION, AND LOCALIZATION IN ATHEROSCLEROTIC LESIONS
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DOI：
10.1073/pnas.87.23.9133
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发表时间：
1990-12-01
影响因子：
11.1
通讯作者：
KODAMA, T
中科院分区：
文献类型：
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作者：
MATSUMOTO, A;NAITO, M;KODAMA, T
从phorbol酯处理的人单核细胞系THP-1 cDNA文库中克隆出两种类型的人巨噬细胞清道夫受体cDNA。由这些cdna编码的I型和II型人类清道夫受体与之前鉴定的牛清道夫受体同源（73%和71%的氨基酸同源），由6个结构域组成：细胞质(I)，膜跨越（II），间隔（III）， α。-螺旋盘状（IV），胶原样(V)和类型特异性c端（VI）。受体基因位于人类第8号染色体上。CHO-K1细胞中表达的人受体介导的修饰低密度脂蛋白内吞作用。在人的肝脏、胎盘和大脑中已检测到两种mrna，分别为4.0和3.2千碱基。免疫组织化学研究使用识别人类清道夫受体的抗肽抗体表明，在富含脂质的动脉粥样硬化病变的巨噬细胞中存在清道夫受体，提示清道夫受体参与动脉粥样硬化的发生。
Two types of cDNAs for human macrophage scavenger receptors were cloned from a cDNA library derived from the phorbol ester-treated human monocytic cell line THP-1. The type I and type II human scavenger receptors encoded by these cDNAs are homologous (73% and 71% amino acid identity) to their previously characterized bovine counterparts and consist of six domains: cytoplasmic (I), membrane-spanning (II), spacer (III), .alpha.-helical coiled-coil (IV), collagen-like (V), and a type-specific C-terminal (VI). The receptor gene is located on human chromosome 8. The human receptors expressed in CHO-K1 cells mediated endocytosis of modified low density lipoproteins. Two mRNAs, 4.0 and 3.2 kilobases, have been detected in human liver, placenta, and brain. Immunohistochemical studies using an anti-peptide antibody which recognizes human scavenger receptors indicated the presence of the scavenger receptors in the macrophages of lipid-rich atherosclerotic lesions, suggesting the involvement of scavenger receptors in atherogenesis.