Cytokine expression in pediatric Helicobacter pylori infection
Cytokine expression in pediatric Helicobacter pylori infection
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DOI：
10.1128/cdli.12.8.994-1002.2005
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发表时间：
2005-08-01
期刊：
影响因子：
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通讯作者：
Fernandes, A
中科院分区：
文献类型：
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作者：
Lopes, AI;Quiding-Jarbrink, M;Fernandes, A
幽门螺杆菌感染是世界范围内最常见的胃肠道感染之一，并且几乎总是引起感染宿主的慢性胃炎。在H.幽门螺杆菌感染的粘膜从成人，但没有以前的研究已经评估了原位细胞因子的表达在儿童。因此，我们通过免疫组化检测了10小时后冷冻保存的胃窦活检标本中促炎、抗炎和调节细胞因子的表达。pylori感染和10个未感染的儿童和相关的细胞因子表达与组织学评分。在8/10例H中观察到白细胞介素-8（IL-8）、γ干扰素（IFN-γ）、IL-4、转化生长因子0和肿瘤坏死因子α的伴随表达。幽门螺杆菌感染病例和5110例非感染病例;幽门感染的受试者显示出至少两种细胞因子的染色。无论是在表面上皮还是腺上皮中，上皮细胞特异性染色的比例在两组之间没有显著差异。此外，各组上皮内或固有层淋巴细胞染色之间无显著差异。然而，在H.幽门感染组。IFN-γ和IL-8固有层淋巴细胞表达与胃窦慢性炎症显著相关，但组织学评分与上皮细胞因子表达无相关性。当使用相同的技术时，H.幽门螺杆菌感染的儿童比成人多，且没有明显的Th 1优势。因此，这些结果表明，在儿童不同的粘膜免疫病理学。H患儿的胃免疫反应是否下调仍有待确定。幽门螺杆菌感染，以及这是否与感染的结果有关。
Helicobacter pylori infection is one of the most common gastrointestinal infections worldwide and almost invariably causes chronic gastritis in the infected host. A predominant Th1 profile has been demonstrated in H. pylori-infected mucosa from adults, but no previous study has evaluated in situ cytokine expression in children. We therefore examined expression of proinflammatory, anti-inflammatory, and regulatory cytokines by immunohistochemistry in cryopreserved antral biopsy specimens from 10 H. pylori-infected and 10 uninfected children and correlated expression of cytokines with histology scores. Concomitant expression of interleukin-8 (IL-8), gamma interferon (IFN-gamma), IL-4, transforming growth factor 0, and tumor necrosis factor alpha was seen in 8/10 H. Pylori-infected cases and in 5110 noninfected cases; all H. pylori-infected subjects showed staining for at least two of the cytokines. The proportion of epithelial cytokine-specific staining did not differ significantly between the groups, either in surface or glandular epithelium. Furthermore, no significant differences were noticed between intraepithelial or lamina propria lymphocyte staining in the groups. There was, however, a tendency of higher numbers of IFN-gamma- and IL-8-positive cells in the H. pylori-infected group. IFN-gamma and IL-8 lamina propria lymphocyte expression correlated significantly with antrum chronic inflammation, but there was no correlation between histology scores and epithelial cytokine expression. When the same techniques were used, the cytokine response appeared to be smaller in H. pylori-infected children than in adults, and there was no clear Th1 dominance. These results therefore suggest a different mucosal immunopathology in children. It remains to be determined whether the gastric immune response is downregulated in children with H. pylori infection and whether this is relevant to the outcome of infection.