Natural variation and CRISPR/Cas9-mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean
Natural variation and CRISPR/Cas9-mediated mutation in GmPRR37 affect photoperiodic flowering and contribute to regional adaptation of soybean
复制标题
GmPRR37 的自然变异和 CRISPR/Cas9 介导的突变影响光周期开花并有助于大豆的区域适应
DOI：
10.1111/pbi.13346
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发表时间：
2020-09-01
影响因子：
13.8
通讯作者：
Han, Tianfu
中科院分区：
文献类型：
--
作者：
Wang, Liwei;Sun, Shi;Han, Tianfu
开花期是大豆地理分布和区域适应性的重要决定因素，受光周期和温度的强烈调控。本研究通过QTL定位和候选基因分析，发现GmPRR 37编码一个假反应调节蛋白，是从极晚花大豆品种‘自贡冬豆’与极早花大豆品种杂交获得的308个重组自交系群体中鉴定出的主效QTL qFT 12 -2的调控基因。'黑河27（HH 27）'，在多种环境中。亲本测序数据的比较分析证实，HH 27含有导致GmPRR 37蛋白中CCT结构域丢失的无义突变。大豆中的CRISPR/Cas9诱导的Gmprr 37-ZGDD突变体在自然长日照（NLD）条件下表现出早花。在长光周期条件下，过表达GmPRR 37的转基因大豆植株开花时间显著延迟。此外，在短日照（SD）条件下，GmPRR 37的敲除和过表达在大豆中均未显示开花时间的显著表型改变。此外，在长日照条件下，GmPRR 37下调促花激素类似物GmFT 2a和GmFT 5a的表达，上调促花激素类似物GmFT 1a的表达。通过对中国180个大豆品种GmPRR 37单倍型的分析，发现天然突变体GmPRR 37开花早，且能使大豆在高纬度地区栽培。本研究表明，GmPRR 37控制大豆光周期开花，并提供了机会，培育优化品种，适应特定的地区和耕作制度。
Flowering time is a critical determinant of the geographic distribution and regional adaptability of soybean (Glycine max) and is strongly regulated by photoperiod and temperature. In this study, quantitative trait locus (QTL) mapping and subsequent candidate gene analysis revealed thatGmPRR37, encoding a pseudo-response regulator protein, is responsible for the major QTLqFT12-2, which was identified from a population of 308 recombinant inbred lines (RILs) derived from a cross between a very late-flowering soybean cultivar, 'Zigongdongdou (ZGDD)', and an extremely early-flowering cultivar, 'Heihe27 (HH27)', in multiple environments. Comparative analysis of parental sequencing data confirmed that HH27 contains a non-sense mutation that causes the loss of the CCT domain in the GmPRR37 protein. CRISPR/Cas9-inducedGmprr37-ZGDD mutants in soybean exhibited early flowering under natural long-day (NLD) conditions. Overexpression ofGmPRR37significantly delayed the flowering of transgenic soybean plants compared with wild-type under long photoperiod conditions. In addition, both the knockout and overexpression ofGmPRR37in soybean showed no significant phenotypic alterations in flowering time under short-day (SD) conditions. Furthermore,GmPRR37down-regulated the expression of the flowering-promotingFThomologuesGmFT2aandGmFT5a, and up-regulated flowering-inhibitingFThomologueGmFT1aexpression under long-day (LD) conditions. We analysed haplotypes ofGmPRR37among 180 cultivars collected across China and found naturalGmprr37mutants flower earlier and enable soybean to be cultivated at higher latitudes. This study demonstrates thatGmPRR37controls soybean photoperiodic flowering and provides opportunities to breed optimized cultivars with adaptation to specific regions and farming systems.