A Multifunctional Cytochrome P450 and a Meroterpenoid Cyclase in the Biosynthesis of Fungal Meroterpenoid Atlantinone B
A Multifunctional Cytochrome P450 and a Meroterpenoid Cyclase in the Biosynthesis of Fungal Meroterpenoid Atlantinone B
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多功能细胞色素 P450 和类萜环化酶在真菌类萜 Atlantinone B 生物合成中的作用
DOI：
10.1021/acs.orglett.2c00684
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发表时间：
2022-04-08
期刊：
影响因子：
5.2
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Qi, Bo-wen;Li, Na;Li, Jun
在产黄青霉MT-40中发现了Atlantinone B（10）的生物合成基因簇。由该簇编码的多功能细胞色素P450（CYP 3D）负责形成独特的内酯桥环和Atlantinone B的16 β-羟基，并且新的萜烯环化酶（CYP 3C）可以前所未有地接受脱甲基底物环氧法尼基-DMOA（4a）以生成三个双环部分萜类化合物（5a-5c）。该研究为组合合成结构多样的单萜类药物奠定了基础。
The biosynthetic gene cluster of atlantinone B (10) was discovered in Penicillium chrysogenum MT-40. A multifunctional cytochrome P450 (AtlD) encoded by the cluster is responsible for the formation of the unique lactone-bridged ring and the 16 beta-hydroxyl of atlantinone B, and a new terpene cyclase (AtlC) can unprecedentedly accept the demethylated substrate epoxyfarnesyl-DMOA (4a) to generate three bicyclic meroterpenoids (5a-5c). This study paves the way for combinatorial synthesis of structurally diverse meroterpenoids for drug discovery.