Rat cerebral cortical synaptoneurosomal membranes. Structure and interactions with imidazobenzodiazepine and 1,4-dihydropyridine calcium channel drugs.
Rat cerebral cortical synaptoneurosomal membranes. Structure and interactions with imidazobenzodiazepine and 1,4-dihydropyridine calcium channel drugs.
复制标题
大鼠大脑皮质突触神经体膜。
DOI：
10.1016/s0006-3495(90)82396-1
复制
发表时间：
1990
影响因子：
3.4
通讯作者：
Herbette,LG
中科院分区：
文献类型：
--
作者：
Moring,J;Shoemaker,WJ;Skita,V;Mason,RP;Hayden,HC;Salomon,RM;Herbette,LG
本文用小角X射线散射研究了大鼠大脑皮层突触神经体膜的结构。制备的电子显微照片显示SNM与经典的突触并置完整，其他囊泡，偶尔线粒体，和一些髓鞘。髓鞘碱性蛋白的免疫测定将正常大鼠SNM的髓鞘含量置于按重量计小于存在的总膜的2%。X-射线衍射图显示了五个衍射级，在较高的水合状态下，膜的晶胞重复为71至78 A。在较低的水化，出现11个订单;晶胞重复是130，表明该晶胞包含两个膜。测定了130-A单位细胞的电子密度分布;它们清楚地显示了SNM囊泡的两个相对的不对称膜。SNM膜/缓冲液分配系数（Kp）的咪唑并苯二氮卓类和1，4-二氢吡啶（DHP）钙通道药物进行了测量; Kp的DHP药物在兔轻肌浆网比SNM高约5倍。Ro 15-1788和DHP BAY K 8644主要与完整SNM膜的囊泡外层单层结合。Ro 15-1788的非特异性平衡结合主要发生在SNM膜脂质提取物中双层的上酰基链。
Small angle x-ray scattering has been used to investigate the structure of synaptoneurosomal (SNM) membranes from rat cerebral cortex. Electron micrographs of the preparation showed SNM with classical synaptic appositions intact, other vesicles, occasional mitochondria, and some myelin. An immunoassay for myelin basic protein placed the myelin content of normal rat SNM at less than 2% by weight of the total membrane present. X-Ray diffraction patterns showed five diffraction orders with a unit cell repeat for the membrane of 71 to 78 A at higher hydration states. At lower hydration, 11 orders appeared; the unit cell repeat was 130 A, indicating that the unit cell contained two membranes. Electron density profiles for the 130-A unit cell were determined; they clearly showed the two opposed asymmetrical membranes of the SNM vesicles. SNM membrane/buffer partition coefficients (Kp) of imidazobenzodiazepine and 1,4-dihydropyridine (DHP) calcium channel drugs were measured; Kp's for DHP drugs were approximately five times higher in rabbit light sarcoplasmic reticulum than in SNM. Ro 15–1788 and the DHP BAY K 8644 bind primarily to the outer monolayer of vesicles of intact SNM membranes. Nonspecific equilibrium binding of Ro 15–1788 occurs mainly in the upper acyl chain of the bilayer in lipid extracts of SNM membrane.
登录
查看更多内容
紫色膜的不对称结构。
影响因子：
56.9
作者：
A. Blaurock;G. I. King
通讯作者：
G. I. King
影响因子：
3.4
作者：
N. Clark;K. Rothschild;D. A. Luippold;B. A. Simon
通讯作者：
B. A. Simon
神经髓磷脂的 X 射线衍射图：确定相位的方法
影响因子：
56.9
作者：
M. F. Moody
通讯作者：
M. F. Moody
使用迭代傅立叶方法确定不对称膜轮廓的结构。
影响因子：
3.4
作者：
R. Stroud;D. Agard
通讯作者：
D. Agard
通过分子力学和定量 X 射线衍射确定胺碘酮在膜双层中的结构和位置。
影响因子：
3.4
作者：
Trumbore,M;Chester,DW;Moring,J;Rhodes,D;Herbette,LG
通讯作者：
Herbette,LG