Effects of Degradable Mg-Ca Alloys on Dendritic Cell Function
Effects of Degradable Mg-Ca Alloys on Dendritic Cell Function
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DOI：
10.1177/0885328209360424
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发表时间：
2011-03-01
影响因子：
2.9
通讯作者：
Bach, F. W.
中科院分区：
文献类型：
--
作者：
Feser, K.;Kietzmann, M.;Bach, F. W.
可降解镁合金是一种用于骨科和创伤外科植入物的新型材料。本研究的目的是研究可降解镁合金对树突状细胞（DC）功能的影响，因为树突状细胞是机体主要的抗原呈递细胞。MgP（纯镁）、MgCa 0.6（0.6%钙）、MgCa 0.8（0.8%钙）、MgCa 1.0（1%钙）和MgCa 1.2（1.2%钙）合金在细胞培养基中降解。同时，小鼠骨髓源性DC分别加入浓度为0.1 ~ 10mmol/L的氯化镁和氯化钙孵育。DC与降解培养基孵育超过6天，对细胞活力没有影响，对DC迁移只有边际影响。此外，与降解镁合金孵育并没有增强TNF α的产生和CD86的表达。混合白细胞反应显示，与未经治疗的对照组相比，t细胞增殖也没有增加。然而，巨噬细胞的发展有以牺牲DC扩张为代价的趋势，并且在较高镁浓度的孵育下可以诱导DC迁移增强。特别是后者需要在体内实验中进行验证。
Degradable magnesium alloys are new materials for implants used in orthopedic and trauma surgery. The aim of this study was to investigate the influence of degradable magnesium alloys on the function of dendritic cells (DC) as these cells represent the major antigen presenting cells of the body. MgP (pure magnesium), MgCa 0.6 (0.6% calcium), MgCa 0.8 (0.8% calcium), MgCa 1.0 (1% calcium), and MgCa 1.2 (1.2% calcium) alloys were degraded in cell culture medium. In parallel, murine bone marrow-derived DC were incubated with increasing concentrations (0.1-10mmol/L) of magnesium chloride and calcium chloride, respectively. Incubation of DC with degradation media over 6 days had no influence on cell viability and only marginal influence on DC migration. Also, the production of TNF alpha and expression of CD86 was not enhanced by incubation with degraded magnesium alloys. The mixed leukocyte reaction revealed that there was also no increase of the T-cell proliferation in comparison to untreated controls. However, there was a trend toward macrophage development at the expense of DC expansion and an enhanced DC migration was induced by incubation with higher magnesium concentrations. Particularly the latter should be verified in in vivo experiments.