Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay
Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay
复制标题
DOI：
10.3109/13693780902977976
复制
发表时间：
2010-03-01
期刊：
影响因子：
2.9
通讯作者：
Koichi, Makimura
中科院分区：
文献类型：
--
作者：
Takashi, Sugita;Miho, Suzuki;Koichi, Makimura
虽然马拉色菌属的亲脂性酵母是健康个体皮肤微生物群的一部分，但它们也与几种皮肤疾病有关，如脂溢性皮炎。然而，年龄和性别对马拉色菌菌群的影响尚未完全阐明。我们分析了770名健康日本人的皮肤马拉色菌微生物群，使用TaqMan探针进行高精度实时PCR，以调查年龄和性别对马拉色菌种群的影响。马拉色菌细胞的数量在男性中增加到16 - 18岁，在女性中增加到10 - 12岁，随后在两种性别中逐渐减少，直到衰老。限制性马拉色菌在16 - 18岁男性和23 - 29岁女性中占绝对优势。M. globosa和M.限制性内切酶占马拉色菌属的70%以上。不分性别的康复。马拉色菌的总定植量和两种主要菌种的比例随年龄和性别而变化。
Although the lipophilic yeasts of the genus Malassezia are part of the cutaneous microbiota in healthy individuals, they are also associated with several skin diseases, such as seborrheic dermatitis. However, the effects of age and gender on the Malassezia microbiota have not been completely elucidated. We analyzed the cutaneous Malassezia microbiota of 770 healthy Japanese using the highly accurate real-time PCR with a TaqMan probe to investigate the effects of age and gender on the Malassezia population. The numbers of Malassezia cells increased in males up to 16-18 years of age and in females to 10-12 years old, and subsequently decreased gradually in both genders until senescence. Malassezia restricta overwhelmingly predominated at ages over 16-18 years in males and 23-29 years in females. M. globosa and M. restricta together accounted for more than 70% of Malassezia spp. recovered regardless of gender. The total colonization of Malassezia and the ratio of the two major species change with age and gender in humans.