Nitrous oxide induces an anxiolytic-like effect in the conditioned defensive burying paradigm, which can be reversed with a benzodiazepine receptor blocker.
Nitrous oxide induces an anxiolytic-like effect in the conditioned defensive burying paradigm, which can be reversed with a benzodiazepine receptor blocker.
复制标题
一氧化二氮在条件性防御性掩埋范式中会产生类似抗焦虑的作用，这种作用可以用苯二氮卓受体阻滞剂逆转。
DOI：
10.1007/bf02245699
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发表时间：
1993
影响因子：
3.4
通讯作者：
Quock,RM
中科院分区：
文献类型：
--
作者：
Czech,DA;Quock,RM
为了研究一氧化二氮 (N2O) 的抗焦虑作用，对雄性戴帽大鼠进行了条件防御性掩埋 (CDB) 测试，该试验利用了大鼠掩埋与厌恶刺激相关的物体的倾向。在接触 N2O 和氧气 (O2) (10-40% N2O) 的四种混合物或室内空气 (RA) 中的一种之前，对接触带电探针的大鼠进行一次短暂的电击。与暴露于 RA 的动物相比，暴露于 N2O 的大鼠表现出与浓度相关的、用地板垫料进行“防御性”掩埋的持续时间和高度的减少；这些措施在 30% N2O 时达到统计显着性。用 20 mg/kg 苯二氮卓受体阻滞剂氟马西尼进行预处理，单独使用没有任何作用，但却有效地拮抗了 N2O 引起的 30% 的埋藏减少。在减弱刺激定向掩埋的 N2O 浓度下，水平运动和站立并没有受到显着影响。使用苯二氮卓类抗焦虑标准药物氯氮卓（2.5-10.0 mg/kg）治疗也会导致埋葬行为的剂量相关减弱。这些发现表明，N2O 可以在该范例中诱导与已知抗焦虑药类似的作用，并表明其介导中存在苯二氮卓机制。
To investigate the anxiolytic effects of nitrous oxide (N2O), male hooded rats were tested in the conditioned defensive burying (CDB) test, a paradigm that exploits a propensity of rats to bury objects associated with aversive stimulation. A single, brief electrical shock was delivered to rats upon contact with an electrified prod, before exposure to one of four mixtures of N2O and oxygen (O2) (10–40% N2O) or room air (RA). Compared to RA-exposed animals, rats exposed to N2O exhibited a concentration-related reduction in duration and height of prod-directed “defensive” burying with floor bedding material; these measures reached statistical significance at 30% N2O. Pretreatment with 20 mg/kg of the benzodiazepine receptor blocker flumazenil, which alone had no effect, effectively antagonized a 30% N2O-induced decrease in burying. Horizontal locomotion and rearing were not significantly affected at concentrations of N2O that attenuated prod-directed burying. Treatment with the benzodiazepine anxiolytic standard, chlordiazepoxide (2.5–10.0 mg/kg) also resulted in dose-related attenuation of burying behavior. These findings show that N2O can induce effects similar to those of known anxiolytics in this paradigm and suggest a benzodiazepine mechanism in its mediation.