Identification and characterization of invertase family genes reveal their roles in vacuolar sucrose metabolism during Pyrus bretschneideri Rehd. fruit development.
Identification and characterization of invertase family genes reveal their roles in vacuolar sucrose metabolism during Pyrus bretschneideri Rehd. fruit development.
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DOI：
10.1016/j.ygeno.2021.01.028
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发表时间：
2021-03
期刊：
影响因子：
4.4
通讯作者：
Suling Zhang;Zhen Zhang;Xun Sun;Zhiqiang Liu;Min Ma;Jinbu Fan;W. Luo;Libin Wang;Shaoling Zhan
中科院分区：
文献类型：
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作者：
Suling Zhang;Zhen Zhang;Xun Sun;Zhiqiang Liu;Min Ma;Jinbu Fan;W. Luo;Libin Wang;Shaoling Zhan
共鉴定出23个转化酶（PbrInvs）基因，包括8个液泡转化酶（PbrvacInvs）、5个细胞壁转化酶（PbrcwInvs）和10个碱性/中性转化酶（PbrA/N-Invs）。bretschneideriRehd。基因组，具有不同的染色体位置，顺式作用元件，基因结构和基序。它们的表达谱具有组织特异性，采后光照或温度处理会改变它们的表达谱。在砀山苏里梨发育过程中，与视觉/内在品质变化相关的是转化酶活性和pbrinvs表达谱的变化。结合亚细胞糖分布以及糖含量、转化酶活性和pbrinvmrna丰度的相关性分析，pbrvacinv1可能参与了梨发育过程中蔗糖的分解。PbrvacInv1-GFP融合蛋白主要聚集在细胞质（液泡膜）上；与此同时，pbrvacinv1在梨果实中的短暂过表达会使vacInv活性上调，导致果实中果糖含量高于对照，蔗糖含量低于对照。此外，转化酶抑制剂5 （PbrInvInh5）可以与PbrvacInv1相互作用。
23invertase(PbrInvs) genes, including eightvacuolar invertases(PbrvacInvs), fivecell wall invertases(PbrcwInvs) and 10alkaline/neutral invertases(PbrA/N-Invs), were identified fromP. bretschneideriRehd. genome, with diverse chromosome locations,cis-acting elements, gene structures and motifs. Their expression profiles were tissue-specific, and postharvest light or temperature treatment would alter their expression profiles. During ‘Dangshansuli’ pear development, in association with visual/inner quality change was the alternations of invertase activity and the expression profiles ofPbrInvs. In combination with results of subcellular sugar distribution as well as correlation analysis among sugar content, invertase activity andPbrInvmRNA abundance,PbrvacInv1might be involved in sucrose decomposition during pear development. PbrvacInv1-GFP fusion protein mainly accumulated on the tonoplast (vacuolar membrane); meanwhile, transient overexpression ofPbrvacInv1in pear fruit would upregulate vacInv activity, causing higher fructose and lower sucrose when compared with that of the control. Furthermore, invertase inhibitor 5 (PbrInvInh5) could interact with PbrvacInv1.