Age-related alterations of Nestin-immunoreactive neurons in rat basal forebrain with aged memory deficit
Age-related alterations of Nestin-immunoreactive neurons in rat basal forebrain with aged memory deficit
复制标题
老年记忆缺陷大鼠基底前脑巢蛋白免疫反应神经元的年龄相关变化
DOI：
10.1016/j.neuint.2008.08.006
复制
发表时间：
2008-12-01
影响因子：
4.2
通讯作者：
Yao, Zhibin
中科院分区：
文献类型：
--
作者：
Li, Dongpei;Wang, Jianmin;Yao, Zhibin
最近的证据表明，不同的Nestin-IR神经元在成年大鼠基底前脑内，Nestin免疫反应（Nestin-IR）神经元的记忆功能障碍的变化进行了研究。Morris水迷宫任务评估了3和24月龄大鼠（年龄受损组和年龄未受损组）的空间学习能力。通过免疫组织化学和阳性神经元计数研究Nestin-IR神经元的分布和形态学特征。Nestin-IR神经元数量随增龄而减少，尤以增龄受损神经元为甚。平均逃避潜伏期与Broca内侧隔-斜角带Nestin-IR神经元数呈显著负相关。吻侧部[内侧隔（MS）和垂直肢斜角带（vDB）]的相关性高于尾侧部[水平肢斜角带（hDB）]。神经元损伤的Nestin-IR神经元树突分支和树突长度的复杂性降低。在部分衰老脑片中，Nestin-IR星形胶质细胞样细胞散在分布于Nestin-IR神经元之间。结论：与老年正常大鼠相比，老年受损大鼠表现出更差的认知空间表现和更少的Nestin-IR神经元数量。Nestin-IR神经元的丢失和形态学改变是老年大鼠基底前脑的一些病理特征，可能是脑老化和老年记忆障碍的重要神经生物学机制。(C)2008爱思唯尔有限公司版权所有。
Age-related and aged memory deficit changes in Nestin-immunoreactive (Nestin-IR) neurons were studied following recent evidence of distinct Nestin-IR neurons within adult rat basal forebrain. Morris water maze task assessed spatial learning capacity of 3- and 24-month rats (aged-impaired and aged-unimpaired groups). Nestin-IR neuron distributional and morphological features were investigated by immunohistochemistry and positive neuronal number calculation. Nestin-IR neuron number declined with aging, especially aged-impaired. Significant negative correlations existed between average escape latencies and Nestin-IR neuron number in medial septum-diagonal band of Broca (MS-DBB). Correlations of rostral portion [medial septum (MS) and vertical limb diagonal band (vDB)] were higher than caudal portion [horizontal limb diagonal band (hDB)]. Aged-impaired showed reduced complexity of Nestin-IR neuron dendrite arborization and dendritic length. Nestin-IR astrocyte-like cells appeared scattered among Nestin-IR neurons oil some aged-impaired slices. In Conclusion, aged-impaired rats showed worse cognitive spatial performance and less Nestin-IR neuronal number compared to aged-Unimpaired. Nestin-IR neuronal loss and morphological changes are some pathological characteristics of rat aged basal forebrain and may be important in neurobiological mechanisms of brain aging and aged memory deficit. (C) 2008 Elsevier Ltd. All rights reserved.