Conditions for the Production of Carotenoids by Thraustochytrium sp. ATCC 26185 and Aurantiochytrium sp. ATCC PRA-276
Conditions for the Production of Carotenoids by Thraustochytrium sp. ATCC 26185 and Aurantiochytrium sp. ATCC PRA-276
复制标题
破囊壶菌生产类胡萝卜素的条件。
DOI：
10.1080/10498850.2019.1603175
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发表时间：
2019
影响因子：
1.6
通讯作者：
C. Cardoso
中科院分区：
文献类型：
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作者：
V. J. Furlan;I. Batista;N. Bandarra;R. Mendes;C. Cardoso
摘要由于类胡萝卜素具有强有力的抗氧化活性，人们一直在寻找生物活性分子的替代来源。一些水生微生物，如破囊壶菌，具有合成大量类胡萝卜素的能力。本调查的目的是研究生长条件（碳：总氮比和培养系统）对类胡萝卜素的生产Thraustochytrium sp.和Aurantiochytrium sp.的影响。对于Thraustochytrium sp.文化，类胡萝卜素的生产降低通过增加初始葡萄糖浓度或连续供应的葡萄糖和氮。对于Aurantiochytrium sp.培养物，限制氮供应或特别是使用葡萄糖和总氮的连续供应降低总类胡萝卜素产量。最高的总类胡萝卜素产量（2.22毫克/升，w/v）后，192小时的培养的破囊壶菌。使用初始浓度为30克/升的葡萄糖和2.4克/升的总氮（分批系统）。最高产量的虾青素实现在流加系统中的C/N比为6.2的破囊壶菌属和40的橙壶菌属。因此，它被发现，类胡萝卜素的配置文件可以通过修改的生长条件，这可以是有用的食品工业和生物技术应用改变。
ABSTRACT There is an intensive search for alternative sources of bioactive molecules, including carotenoids, due to their potent antioxidant activities. Some aquatic microorganisms, such as thraustochytrids, have the ability to synthesize large amounts of carotenoids. The objective of this investigation was to study the effect of growth conditions (carbon: total nitrogen ratio and cultivation system) on the production of carotenoids by Thraustochytrium sp. and Aurantiochytrium sp. For Thraustochytrium sp. culture, carotenoid production was lowered by increasing the initial glucose concentration or continuous supply of glucose and nitrogen. For Aurantiochytrium sp. culture, restraining nitrogen supply or especially using a continuous supply of glucose and total nitrogen decreased total carotenoid productivity. The highest total carotenoid production (2.22 mg/L, w/v) was obtained after 192 h of cultivation of Thraustochytrium sp. using initial concentrations of 30 g/L glucose and 2.4 g/L total nitrogen (batch system). The highest production of astaxanthin was achieved in the fed-batch system with a C/N ratio of 6.2 for Thraustochytrium sp. and 40 for Aurantiochytrium sp. Therefore, it was found that the carotenoid profile can be changed by modifying the growth conditions, which can be useful for the food industry and biotechnological applications.