Comparative transcriptome profiling of potassium starvation responsiveness in two contrasting watermelon genotypes
Comparative transcriptome profiling of potassium starvation responsiveness in two contrasting watermelon genotypes
复制标题
两种对比西瓜基因型钾饥饿反应的比较转录组分析
DOI：
10.1007/s00425-013-1976-z
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发表时间：
2014-02-01
期刊：
影响因子：
4.3
通讯作者：
Bie, Zhilong
中科院分区：
文献类型：
--
作者：
Fan, Molin;Huang, Yuan;Bie, Zhilong
钾是作物必需的营养元素之一，缺钾严重制约着作物的产量和品质。西瓜[Citrulluslanatus（Thunb.）马特森& Nakai]是一种重要的经济作物，经常遭受K+缺乏。为阐明西瓜耐低钾的机理，提高西瓜及其他作物的钾效率，以两个钾效率差异较大的西瓜基因型YS和8424为材料，比较了它们对低钾胁迫的反应机制。YS比8424更耐K+胁迫，根系生长受抑制的程度更小。在处理后6和120 h（HAT）收获YS和8424幼苗的根（有或没有K+供应），并通过Illumina RNA测序分析其转录组。不同的根K+吸收基因的短期和长期胁迫的调节机制进行了观察。参与茉莉酸和活性氧产生的基因; Ca 2+和受体样激酶信号传导;木质素生物合成;和其他应激相关基因在YS中被抑制，而大量的这种应激相关基因在8424中在120 HAT下被诱导。这些结果表明，抑制防御和胁迫反应可以节省能量，使YS根系生长更好，从而促进K+吸收，提高K+效率和对K+缺乏的耐性。本研究首次在西瓜中发现了全局根转录组，并为钾高效西瓜基因型耐钾缺乏的分子机制提供了新的见解。
Potassium (K) is one of the essential nutrients for crops, and K+deficiency highly restricts crop yield and quality. Watermelon [Citrulluslanatus(Thunb.) Matsum. & Nakai] is an economically important crop that often suffers from K+deficiency. To elucidate the underlying tolerance mechanism of watermelon to K+deficiency and to improve K efficiency of watermelon and other crops in the future, two watermelon genotypes, namely, YS and 8424, that exhibit contrasting K efficiencies were studied to compare their response mechanisms to K+deficiency. YS was more tolerant of K+deficiency and displayed less inhibited root growth than 8424. Roots of YS and 8424 seedlings with or without K+supply were harvested at 6 and 120 h after treatment (HAT), and their transcriptomes were analyzed by Illumina RNA sequencing. Different regulation mechanisms of the root K+-uptake genes for short- and long-term stress were observed. Genes involved in jasmonic acid and reactive oxygen species production; Ca2+and receptor-like kinase signaling; lignin biosynthesis; and other stress-related genes were repressed in YS, whereas a large number of such stress-related genes were induced in 8424 at 120 HAT. These results suggested that repressed defense and stress response can save energy for better root growth in YS, which can facilitate K+uptake and increase K efficiency and tolerance to K+deficiency. This study presents the first global root transcriptome in watermelon and provides new insights into the molecular mechanisms underlying tolerance to K+deficiency of K-efficient watermelon genotypes.