SecA protein is directly involved in protein secretion in Escherichia coli
SecA protein is directly involved in protein secretion in Escherichia coli
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SecA蛋白直接参与大肠杆菌的蛋白质分泌
DOI：
10.1016/0014-5793(89)80516-2
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发表时间：
1989
期刊：
影响因子：
3.5
通讯作者：
S. Mizushima
中科院分区：
文献类型：
--
作者：
H. Kawasaki;S. Matsuyama;S. Sasaki;M. Akita;S. Mizushima
构建了该基因的高表达质粒。SecA蛋白随后过量生成inE。coliand净化。纯化的SecA刺激模型分泌蛋白在体外易位到4 M尿素预处理的倒置膜泡中。来自SecA突变体的膜囊泡表现出较低的易位活性，而SecA增强了这一活性。这些结果表明SecA直接参与细胞质膜蛋白的分泌。
A high-expression plasmid for thesecAgene was constructed. The SecA protein was then overproduced inE. coliand purified. The purified SecA stimulated the in vitro translocation of a model secretory protein into inverted membrane vesicles pretreated with 4 M urea. Membrane vesicles from a secAtsmutant exhibited lower translocation activity, which was enhanced by SecA. These results indicate that SecA is directly involved in protein secretion across the cytoplasmic membrane.