On the development of ectopic eyes in Drosophila melanogaster produced by the mutation extra eye (ee).
On the development of ectopic eyes in Drosophila melanogaster produced by the mutation extra eye (ee).
复制标题
果蝇异位眼的发育是由突变额外眼（ee）产生的。
DOI：
10.1093/genetics/111.1.67
复制
发表时间：
1985
期刊：
影响因子：
3.3
通讯作者：
M. C. McElwain
中科院分区：
文献类型：
--
作者：
W. K. Baker;D. Marcey;M. C. McElwain
突变ee通常在头部顶点产生异位眼，这是头部同侧正常眼的镜像部分复制。复制模式和有丝分裂重组克隆分析表明，复制为眼背和眶背结构。大异位眼（超过100个小眼）及其周围的刚毛可以在没有角质层缺陷的情况下产生。ee的外显率与温度有关，25度和29度的外显率（72%）高于19度（43%）。温度变化实验显示了两个温度敏感期：一个在中期发生，另一个在第一幼虫中期。二龄晚期和三龄影像头盘的显微镜检查显示，直到三龄中期才有产生复制所需的额外组织生长的迹象。这是证实了细胞计数的ee和野生型盘。在这一阶段，没有证据表明两种类型的椎间盘有不同的细胞死亡，尽管假设细胞死亡要早得多。通过产生形态发生克隆来测试突变的细胞自主性表明非自主性。本文讨论了突变基因模式复制的形成，包括消除整个发育区室的细胞死亡、发生在一个区室内的有限细胞死亡、发生在一个区室内的广泛细胞死亡以及与细胞致命性无关的增殖生长。
The mutation ee often produces an ectopic eye on the vertex that is a mirror image partial duplication of the normal eye on the ipsilateral side of the head. The pattern of the duplication and a clonal analysis by mitotic recombination indicate that the duplications are of dorsal eye and orbital structures. Large ectopic eyes (more than 100 ommatidia) and their surrounding bristles may be produced without cuticular deficiencies. The penetrance of ee is temperature dependent with penetrance higher (72%) at 25 degrees and 29 degrees than at 19 degrees (43%). Temperature shift experiments show two temperature-sensitive periods: one at midembryogenesis, the other at mid-first larval instar. Microscopic examination of ee late-second and third instar imaginal cephalic discs show no indication of growth of the extra tissue needed to produce the duplication until after mid-third instar. This was confirmed by cell counts of ee and wild-type discs. There is no evidence of differential cell death in the two types of discs at this stage, although much earlier cell death is postulated. Tests for cell autonomy of the mutation by the production of morphogenetic clones suggest nonautonomy. Formation of pattern duplications by mutant genes is discussed in terms of cell death that eliminates whole developmental compartments, restricted cell death that occurs within a compartment, extensive cell death within a compartment and proliferative growth unassociated with cell lethality.