Antibody-directed liposomes: the development of a cell-specific cytotoxic agent.
Antibody-directed liposomes: the development of a cell-specific cytotoxic agent.
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抗体导向脂质体：细胞特异性细胞毒剂的开发。
DOI：
10.1042/bst0120340
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发表时间：
1984
影响因子：
3.9
通讯作者：
Papahadjopoulos,D
中科院分区：
文献类型：
--
作者：
Heath,TD;Bragman,KS;Matthay,KK;Lopez-Straubinger,NG;Papahadjopoulos,D
脂质体经常被提议用作药物载体（Mayhew & papahadjoopoulos, 1983）。脂质体在其内部水空间或脂质双分子层内包封药物的能力使其成为一种多用途的载体系统，可以通过两种方式之一提高药物的功效。首先，作为一个缓释系统，脂质体可以改变药物的药代动力学，从而延长有效的血浆药物水平。其次，脂质体作为一种微观载体系统，可以将药物直接运送到细胞中。第二种方法的成功使用首先需要脂质体被特异性地引导到目标细胞，其次，它们能够在到达细胞后将被封装的药物传递给细胞。这两个特征都可以通过体外实验来检验，我们将在这里回顾我们为实现这些目标而进行的研究。脂质体针对特定细胞类型的最容易实现的方法是将配体附着在脂质体表面，脂质体将与靶细胞质膜上的决定因子结合。可用的最通用的配体是抗体，它可以广泛地产生
Liposomes have often been proposed for use as drug carriers (Mayhew & Papahadjopoulos, 1983). The ability of liposomes to encapsulate drugs within their internal aqueous space or in the lipid bilayer makes them a versatile carrier system which may improve the efficacy of drugs in one of two ways. First, by acting as a slow release system, liposomes may modify the pharmacokinetics of drugs so as to prolong effective plasma drug levels. Second, being a microscopic carrier system, liposomes may transport and deliver drugs directly to cells. The successful use of the second approach requires first that liposomes be directed specifically to the target cell, and second that they be capable of delivering the encapsulated drug to the cells once they reach them. Both of these characteristics can be examined by experiments in uitro, and we will here review our investigations which have been carried out to achieve these aims.The targeting of liposomes to specific cell types is most readily achieved by attaching ligands to the liposome surface which will bind to determinants on the plasma membrane of the target cell. The most versatile ligands available are antibodies which can be produced to a wide