High-performance liquid chromatographic enantioseparations on capillary columns containing crosslinked polysaccharide phenylcarbamate derivatives attached to monolithic silica
High-performance liquid chromatographic enantioseparations on capillary columns containing crosslinked polysaccharide phenylcarbamate derivatives attached to monolithic silica
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DOI：
10.1002/jssc.200500388
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发表时间：
2006-08-01
影响因子：
3.1
通讯作者：
Okamoto, Yoshio
中科院分区：
文献类型：
--
作者：
Chankvetadze, Bezhan;Kubota, Takateru;Okamoto, Yoshio
用纤维素和直链淀粉的3，5-二取代苯基氨基甲酸酯衍生物共价修饰天然硅胶整体毛细管柱，并将其应用于毛细管液相色谱中对映体分离。先前用于共价固定化多糖苯基氨基甲酸酯衍生物到微粒硅胶表面上的方法成功地适用于整体熔融石英毛细管柱的原位改性。研究了多糖的性质、取代基以及多糖衍生物的多重共价固定对毛细管柱色谱性能的影响。用该技术制备的毛细管柱在所有常用溶剂中均稳定，并表现出良好的对映体拆分能力。
Monolithic capillary columns containing native silica gel were covalently modified with 3,5-disubstituted phenylcarbamate derivatives of cellulose and amylose and applied for enantioseparations in capillary LC. The method previously used for covalent immobilization of polysaccharide phenylcarbamate derivatives onto the surface of microparticulate silica gel was successfully adapted for in situ modification of monolithic fused-silica capillary columns. The effects of the nature of polysaccharide and the substituents, as well as of multiple covalent immobilization of polysaccharide derivative on chromatographic performance of capillary columns were studied. The capillary columns obtained using this technique are stable in all solvents commonly used in LC and exhibit promising enantiomer resolving ability.