The 1H, 13C and 15N backbone and side-chain assignment of the RRM domain of SC35, a regulator of pre-mRNA splicing.
The 1H, 13C and 15N backbone and side-chain assignment of the RRM domain of SC35, a regulator of pre-mRNA splicing.
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SC35（前体 mRNA 剪接调节因子）RRM 结构域的 1H、13C 和 15N 主链和侧链分配。
DOI：
10.1007/s12104-010-9254-5
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发表时间：
2011
影响因子：
0.9
通讯作者：
Clayton JC
中科院分区：
文献类型：
--
作者：
Clayton JC
富含丝氨酸-精氨酸的蛋白质家族在组成性剪接和选择性剪接的调节中起重要作用。SC 35（也称为SFRS 2和PR 264）是该家族的成员，包含一个RNA识别基序（RRM结构域）和一个富含精氨酸和丝氨酸残基的C-末端RS结构域。SC 35特异性参与细胞增殖和细胞周期进程的主要调控途径。确定SC 35的结构将有助于更好地了解其结构与其许多功能的关系。对SC 35的RRM结构域进行了1H、13 C和15 N的完全赋值。使用均匀的[15 N，13 C]-标记的蛋白质的2D异质结和3D三重共振实验获得的分配。化学位移被用来预测在RNA的情况下，这个RRM域的三维结构。
The serine-arginine rich family of proteins play important roles in the regulation of both constitutive and alternative splicing. SC35 (also known as SFRS2 and PR264) is a member of this family and contains one RNA recognition motif (RRM domain) and a RS domain at the C-terminus which is enriched with arginine and serine residues. SC35 is specifically involved in major regulatory pathways for cell proliferation and cell cycle progression. Determining the structure of SC35 would enable greater understanding of how its structure relates to its many functions. Complete1H,13C and15N assignments of the RRM domain of SC35 are presented. The assignments were obtained using 2D heteronuclear and 3D triple-resonance experiments with the uniformly [15N,13C]-labelled protein. The chemical shifts are used to predict the 3-dimensional structure of this RRM domain in the absence of RNA.