Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs.
Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs.
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DOI：
10.1073/pnas.82.9.2834
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发表时间：
1985-05
影响因子：
11.1
通讯作者：
D. Wells;L. Kedes
中科院分区：
文献类型：
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作者：
D. Wells;L. Kedes
我们从人成纤维细胞cDNA文库中分离并测序了编码人基础表达H3.3组蛋白的全长cDNA克隆。这种非典型cDNA的几个特征将其和其基因与充分表征的细胞周期调控组蛋白基因及其RNA转录物区分开来。H3.3 mRNA约等于1200个碱基长，含有异常长的5'和3'非翻译区，并具有3'聚腺苷酸化末端。此外，我们已经分离和表征了一个cDNA克隆，它是H3.3 mRNA的前体，含有一个中断其5'非翻译区的插入序列。cDNA亚片段与人类基因组DNA的杂交揭示了一个复杂的多基因家族。基础组蛋白基因和细胞周期组蛋白基因的结构差异表明了一个解释其表达差异的模型。
We have isolated and sequenced full-length cDNA clones encoding the human basally expressed H3.3 histone from a human fibroblast cDNA library. Several features of this atypical cDNA distinguish it and its gene from the well-characterized cell-cycle regulated histone genes and their RNA transcripts. The H3.3 mRNA is approximately equal to 1200 bases long, contains unusually long 5' and 3' untranslated regions, and has a 3' polyadenylylated terminus. In addition, we have isolated and characterized a cDNA clone that is a precursor to the H3.3 mRNA and contains an intervening sequence interrupting its 5' untranslated region. Hybridization of subsegments of the cDNA to human genomic DNA reveals a complex multigene family. The differences in the structures of basal and cell-cycle histone genes suggest a model to explain the differences in their expression.