Vertical Structure of Bacterial and Archaeal Communities within the Sediment of a Eutrophic Lake as Revealed by Culture-Independent Methods
Vertical Structure of Bacterial and Archaeal Communities within the Sediment of a Eutrophic Lake as Revealed by Culture-Independent Methods
复制标题
通过独立于培养的方法揭示富营养化湖泊沉积物内细菌和古菌群落的垂直结构
DOI：
10.1080/02705060.2010.9664406
复制
发表时间：
2010-12-01
影响因子：
1.3
通讯作者：
Yang, Liuyan
中科院分区：
文献类型：
--
作者：
Liu, Lu;Peng, Yuke;Yang, Liuyan
摘要采用16 SrDNA变性梯度凝胶电泳和荧光原位杂交技术，对太湖沉积物中细菌群落组成、主要细菌系统发育类群古菌的分布及功能类群进行了研究。微生物群落随沉积物深度的变化而变化。细菌占DAPI（4′，6′-diamidino-2-phenylindole）细胞总数的30-50%，而古细菌占总细胞数的5-10%。β-变形菌在上层沉积物中含量最高，占总DAPI细胞数的10-15%，在下层沉积物中γ-变形菌占总DAPI细胞数的11%。蓝藻占总细胞数的4-18%，在沉积物中检测到的深度达45厘米。在氮转化功能群中，推测的氨氧化菌群数量随深度的增加而增加，而氨氧化菌群数量随深度的增加而减少，表明沉积物具有氮转化能力。
ABSTRACT The bacterial community composition and distribution of major bacterial phylogenetic groups, archaea, as well as the functional groups, from sediment of Lake Taihu were investigated by denaturing gradient gel electrophoresis of amplified 16S rDNA and fluorescence in situ hybridization. The microbial community changed with depth of sediments. Up to 30–50% of total DAPI (4′, 6′-diamidino-2-phenylindole) cell counts were bacteria, while archaea reached as high as 5–10% of the total cell counts. β-Proteobacteria was the most abundant group detected in upper sediment, accounting for up to 10–15% of total DAPI cell counts, and up to 11% of total DAPI cell counts belonged to y-Proteobacteria in lower sediment. Cyanobacteria accounted for up to 4–18% of total cell counts and were detected as deep as 45 cm in the sediment. For the nitrogen transformation functional groups, putative ammonia oxidation Archaea—Crenarchaeota counts increased with the depth, while the ammonia oxidation bacteria decreased with the depth, which indicated the nitrogen transformation ability in the sediments.