Stochastic yet biased expression of multiple Dscam splice variants by individual cells
Stochastic yet biased expression of multiple Dscam splice variants by individual cells
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DOI：
10.1038/ng1299
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发表时间：
2004-03-01
期刊：
影响因子：
30.8
通讯作者：
Chess, A
中科院分区：
文献类型：
--
作者：
Neves, G;Zucker, J;Chess, A
黑腹果蝇的基因Dscam对轴突的引导至关重要，有38,016种可能的剪接形式。这种多样性可能被用来区分细胞。我们分析了不同细胞类型和单个细胞表达的Dscam mRNA亚型。表达的剪接变体的选择在空间和时间上都受到调控。不同的光感受器亚型表达广泛而独特的Dscam亚型。单细胞RT-PCR记录了单个细胞表达几种不同的Dscam亚型，并允许对存在的多样性进行估计。例如，我们估计每个R3/R4感光细胞表达14-50种不同的mrna，这些mrna是从其细胞类型的数千种剪接变体中选择出来的。因此，每个细胞的Dscam库与其相邻的细胞不同，提供了在神经系统和其他地方产生独特细胞身份的潜在机制。
The Drosophila melanogaster gene Dscam is essential for axon guidance and has 38,016 possible alternative splice forms. This diversity can potentially be used to distinguish cells. We analyzed the Dscam mRNA isoforms expressed by different cell types and individual cells. The choice of splice variants expressed is regulated both spatially and temporally. Different subtypes of photoreceptors express broad yet distinctive spectra of Dscam isoforms. Single-cell RT-PCR documented that individual cells express several different Dscam isoforms and allowed an estimation of the diversity that is present. For example, we estimate that each R3/R4 photoreceptor cell expresses 14-50 distinct mRNAs chosen from the spectrum of thousands of splice variants distinctive of its cell type. Thus, the Dscam repertoire of each cell is different from those of its neighbors, providing a potential mechanism for generating unique cell identity in the nervous system and elsewhere.