CELL-CYCLE EFFECTS AND CELLULAR PHARMACOLOGY OF 5-AZA-2'-DEOXYCYTIDINE
CELL-CYCLE EFFECTS AND CELLULAR PHARMACOLOGY OF 5-AZA-2'-DEOXYCYTIDINE
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DOI：
10.1007/bf00269027
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发表时间：
1984-01-01
影响因子：
3
通讯作者：
RIVARD, GE
中科院分区：
文献类型：
--
作者：
MOMPARLER, RL;SAMSON, J;RIVARD, GE
研究了 5-aza-2''-脱氧胞苷 (5-AZA-CdR) [一种非常有效的抗白血病剂] 在 EMT6 鼠 [乳腺] 肿瘤细胞的同步化细胞以及对数期和平台期培养物中的细胞毒性作用。 5-AZA-CdR 对 S 期细胞产生比 G1 期细胞更大的细胞杀伤力。生长对数期的细胞比生长平台期的细胞对 5-AZA-CdR 的细胞毒性作用更敏感。 5-AZA-CdR 优先杀死细胞周期 S 期的细胞。 5-AZA-CdR不阻断细胞进入S期的细胞周期进程。暴露于 5-AZA-CdR 的 EMT6 细胞的存活曲线表明该类似物的细胞毒性作用不是自限性的。通过诱导中国仓鼠卵巢细胞的 6-硫鸟嘌呤抗性来研究 5-AZA-CdR 的诱变活性。 5-AZA-CdR 在此测定系统中不是可检测到的诱变剂。
The cytotoxic action of 5-aza-2''-deoxycytidine (5-AZA-CdR) [a very potent antileukemic agent] in synchronized cells and logarithmic- and plateau-phase cultures of EMT6 murine [mammary] tumor cells was investigated. 5-AZA-CdR produced a greater cell kill of S phase cells than of cells in G1 phase. Cells in the logarithmic phase of growth were more sensitive to the cytotoxic effects of 5-AZA-CdR than cells in the plateau phase of growth. 5-AZA-CdR produces a preferential kill of cells in the S phase of the cell cycle. 5-AZA-CdR did not block the cell cycle progression of cells into S phase. The survival curve of EMT6 cells exposed to 5-AZA-CdR suggests that the cytotoxic action of this analog is not self-limiting. The mutagenic activity of 5-AZA-CdR was investigated using induction of 6-thioguanine resistance in Chinese hamster ovary cells. 5-AZA-CdR was not a detectable mutagen in this assay system.