Functional recovery in acute traumatic spinal cord injury after transplantation of human umbilical cord mesenchymal stem cells
Functional recovery in acute traumatic spinal cord injury after transplantation of human umbilical cord mesenchymal stem cells
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DOI：
10.1097/ccm.0b013e3181f17c0e
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发表时间：
2010-11-01
影响因子：
8.8
通讯作者：
Feng, Hua
中科院分区：
文献类型：
--
作者：
Hu, Sheng-Li;Luo, Hai-Shui;Feng, Hua
目的：脊髓损伤可导致神经元丢失、轴突变性、胶质瘢痕形成和严重的功能障碍。人脐带间充质干细胞在体外可诱导分化为神经细胞。因此，这些细胞对治疗脊髓损伤具有潜在的治疗作用。设计和设置：将大鼠随机分为三组：假手术组、对照组和人脐带间充质干细胞组。除假手术组外，其余各组均采用失重法造成脊髓损伤。对照组接受Dulbecco改良的必需培养基/营养混合物F-12注射，而人脐带间充质干细胞组在损伤后24小时在脊髓背侧向损伤部位头侧2 mm和尾侧2 mm处进行细胞移植，测量：检测各组大鼠的神经功能和脑源性神经营养因子、胶质细胞系源性神经营养因子和神经营养素-3的含量。存活，迁移，和人脐带间充质干细胞的分化，轴突再生，和胶质瘢痕的形成也探讨了通过使用免疫组化和免疫荧光。主要结果：后肢运动功能的恢复显着增强人脐带间充质干细胞移植动物在移植后5周。这种恢复伴随着神经元阳性纤维长度的增加和病变部位周围生长锥样结构数量的增加。移植的人脐带间充质干细胞存活，迁移短距离，并产生大量的胶质细胞源性神经营养因子和神经营养素3在宿主脊髓。在人脐带间充质干细胞组中，脊髓头端和尾端的反应性星形胶质细胞比对照组少。结论：用人脐带间充质干细胞治疗可以促进创伤性脊髓损伤后的功能恢复，并可能被证明是一种有用的治疗策略，以修复损伤的脊髓。(Crit Care Med 2010; 38：2181-2189）
Objective: Spinal cord injury results in loss of neurons, degeneration of axons, formation of glial scar, and severe functional impairment. Human umbilical cord mesenchymal stem cells can be induced to form neural cells in vitro. Thus, these cells have a potential therapeutic role for treating spinal cord injury.Design and Setting: Rats were randomly divided into three groups: sham operation group, control group, and human umbilical cord mesenchymal stem cell group. All groups were subjected to spinal cord injury by weight drop device except for sham group.Subjects: Thirty-six female Sprague-Dawley rats.Interventions: The control group received Dulbecco's modified essential media/nutrient mixture F-12 injections, whereas the human umbilical cord mesenchymal stem cell group undertook cells transplantation at the dorsal spinal cord 2 mm rostrally and 2 mm caudally to the injury site at 24 hrs after spinal cord injury.Measurements: Rats from each group were examined for neurologic function and contents of brain-derived neurotrophic factor, glial cell line-derived neurotrophic factor, and neurotrophin-3. Survival, migration, and differentiation of human umbilical cord mesenchymal stem cells, regeneration of axons, and formation of glial scar were also explored by using immunohistochemistry and immunofluorescence.Main Results: Recovery of hindlimb locomotor function was significantly enhanced in the human umbilical cord mesenchymal stem cells grafted animals at 5 wks after transplantation. This recovery was accompanied by increased length of neurofilament-positive fibers and increased numbers of growth cone-like structures around the lesion site. Transplanted human umbilical cord-mesenchymal stem cells survived, migrated over short distances, and produced large amounts of glial cell line-derived neurotrophic factor and neurotrophin-3 in the host spinal cord. There were fewer reactive astrocytes in both the rostral and caudal stumps of the spinal cord in the human umbilical cord-mesenchymal stem cell group than in the control group.Conclusions: Treatment with human umbilical cord mesenchymal stem cells can facilitate functional recovery after traumatic spinal cord injury and may prove to be a useful therapeutic strategy to repair the injured spinal cord. (Crit Care Med 2010; 38: 2181-2189)