Caspases and programmed cell death in the hypersensitive response of plants to pathogens
Caspases and programmed cell death in the hypersensitive response of plants to pathogens
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DOI：
10.1016/s0960-9822(98)70469-5
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发表时间：
1998-10-08
期刊：
影响因子：
9.2
通讯作者：
Lam, E
中科院分区：
文献类型：
--
作者：
del Pozo, O;Lam, E
过敏性反应(HR)是由某些植物病原体诱导的，涉及程序性细胞死亡(PCD)，以限制病原体从侵染部位的传播[1]。在诱导细胞死亡的同时，宿主激活了防御反应[2]。在几个植物病原体系统中，与HR相关的细胞死亡在形态上与动物的凋亡相似[3，4]，这表明植物和动物的细胞死亡机制可能具有共同的成分，导致相似的细胞事件。Caspase是一种保守的半胱氨酸蛋白酶，调节动物的PCD[5]；caspase活性或caspase参与细胞死亡在植物中尚未有报道。在这项工作中，我们研究了caspase在HR细胞死亡中的作用。Caspase特异性多肽抑制剂Ac-YVAD-CMK[6]和Ac-DEVD-CHO[7]可以消除细菌诱导的植物PCD，但不显著影响HR的其他方面的诱导，如防御基因的表达。这一结果证实了我们之前的模型，即在HR过程中，细胞死亡可以从防御基因的激活中分离出来[8]。在感染烟草花叶病毒(TMV)后发生HR的烟草组织中检测到Caspase样蛋白水解酶活性。我们的结果为参与HR细胞死亡的半胱氨酸蛋白酶样植物蛋白酶(S)的存在提供了证据。(C)现代生物有限公司ISSN 0960-9822。
The hypersensitive response (HR) is induced by certain plant pathogens and involves programmed cell death (PCD) to restrict the spread of pathogens from the infection site [1]. Concurrent with the induction of cell death, the host activates a defense response [2]. The cell death associated with the HR in several plant-pathogen systems has morphological similarities to animal apoptosis [3,4], which suggests that cell death mechanisms in plants and animals may share common components that lead to similar cellular events. Caspases are conserved cysteine proteases that regulate animal PCD [5]; caspase activity or an involvement of caspases in cell death has yet to be reported in plants. In this work, we investigated the participation of caspases in HR cell death. Caspase-specific peptide inhibitors, Ac-YVAD-CMK [6] and Ac-DEVD-CHO [7], could abolish bacteria-induced plant PCD but did not significantly affect the induction of other aspects of HR, such as the expression of defense genes. This result confirmed our previous model that cell death can be uncoupled from defense gene activation during HR [8]. Caspase-like proteolytic activity was detected in tobacco tissues that were developing HR following infection with tobacco mosaic virus (TMV). Our results provide evidence for the presence of caspase-like plant protease(s) that participate in HR cell death. (C) Current Biology Ltd ISSN 0960-9822.