Evaluation of the Gastrointestinal Tract as Potential Route of Primary Polyomavirus Infection in Mice.
Evaluation of the Gastrointestinal Tract as Potential Route of Primary Polyomavirus Infection in Mice.
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胃肠道作为小鼠原发性多瘤病毒感染潜在途径的评估
DOI：
10.1371/journal.pone.0150786
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Randhawa P
中科院分区：
文献类型：
--
作者：
Huang G;Zeng G;Huang Y;Ramaswami B;Randhawa P
在世界各地的大城市河流中检测到多瘤病毒(PYV)DNA，表明初级病毒感染可通过口腔途径发生。这项研究的目的是通过实验来检验这一概念。用小鼠PYV(MPyV)经鼻或经胃感染C57BL/6J小鼠。用定量聚合酶链式反应研究病毒感染后3、7、10、14、21和28天的病毒载量动态变化。鼻感染后，MPyV DNA在肺、心脏、主动脉、结肠和粪便等器官中很容易检测到，病毒载量在103-106拷贝/mg湿重范围内，最高可达7-10dpi。在28dpi时，病毒在血清和肾脏中完全清除，而在其他器官中的清除是部分的，病毒载量减少了10-100倍。相比之下，在胃内感染后，PYV的峰值检测延迟到21dpi，病毒载量最多降低3logs。在心脏、结肠或大便中没有检测到病毒。胃内感染MPyV的途径是成功的，但不如呼吸道途径有效，而且与病毒延迟传播以及个别器官的MPyV载量峰值较低有关。
Detection of Polyomavirus (PyV) DNA in metropolitan rivers worldwide has led to the suggestion that primary viral infection can occur by the oral route. The aim of this study was to test this notion experimentally. Mouse PyV (MPyV) was used to infect C57BL/6J mice by the nasal or intragastric route. Viral load kinetics was studied 3, 7, 10, 14, 21 and 28 days post-infection (dpi) using quantitative PCR. Following nasal infection, MPyV DNA was readily detected in many organs including lung, heart, aorta, colon, and stool with viral loads in the range of 103–106 copies/mg wet weight that peaked 7–10 dpi. Complete viral clearance occurred in the serum and kidney by 28 dpi, while clearance in other organs was partial with a 10–100 fold decrease in viral load. In contrast, following intragastric infection peak detection of PyV was delayed to 21 dpi, and viral loads were up to 3 logs lower. There was no detectable virus in the heart, colon, or stool. The intragastric route of MPyV infection is successful, not as efficacious as the respiratory route, and associated with delayed viral dissemination as well as a lower peak MPyV load in individual organs.