The glmS riboswitch integrates signals from activating and inhibitory metabolites in vivo.
The glmS riboswitch integrates signals from activating and inhibitory metabolites in vivo.
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DOI：
10.1038/nsmb.1989
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发表时间：
2011-03
影响因子：
16.8
通讯作者：
中科院分区：
文献类型：
--
作者：
glmS核糖开关属于调节RNA家族，其提供代谢基因的反馈调节。它也是一种核酶，在结合葡萄糖胺-6-磷酸（glmS编码的酶的产物）后自切割。第一次在酵母模型系统中测量了细胞内自切割动力学的配体浓度依赖性，并且令人惊讶地揭示了这种核糖开关受到己糖代谢物的抑制和激活。B中的报告基因实验。枯草芽孢杆菌证实，这种核糖开关在其天然生物环境中整合了正和负化学信号。与传统的核糖开关只对单一同源代谢物做出反应的观点相反，我们的新模型提出，单个核糖开关整合了来自一系列化学信号的信息，以根据细胞的整体代谢状态来调节基因表达。
The glmS riboswitch belongs to the family of regulatory RNAs that provide feedback regulation of metabolic genes. It is also a ribozyme that self-cleaves upon binding glucosamine-6-phosphate, the product of the enzyme encoded by glmS. The ligand concentration dependence of intracellular self-cleavage kinetics was measured for the first time in a yeast model system and surprisingly revealed that this riboswitch is subject to inhibition as well as activation by hexose metabolites. Reporter gene experiments in B. subtilis confirmed that this riboswitch integrates positive and negative chemical signals in its natural biological context. Contrary to the conventional view that a riboswitch responds to just a single cognate metabolite, our new model proposes that a single riboswitch integrates information from an array of chemical signals to modulate gene expression according to the overall metabolic state of the cell.
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影响因子：
2.7
作者：
Geiser, M;Cèbe, R;Schmitz, R
通讯作者：
Schmitz, R
DOI：
10.1073/pnas.91.15.6977
发表时间：
1994-07-19
影响因子：
11.1
作者：
LONG, DM;UHLENBECK, OC
通讯作者：
UHLENBECK, OC
影响因子：
4.1
作者：
BATES, CJ;PASTERNAK, CA
通讯作者：
PASTERNAK, CA
影响因子：
5.4
作者：
Dambach, Michael D.;Winkler, Wade C.
通讯作者：
Winkler, Wade C.
影响因子：
56.9
作者：
Klein, Daniel J.;Ferre-D'Amare, Adrian R.
通讯作者：
Ferre-D'Amare, Adrian R.