The RNA expression signature of the HepG2 cell line as determined by the integrated analysis of miRNA and mRNA expression profiles
The RNA expression signature of the HepG2 cell line as determined by the integrated analysis of miRNA and mRNA expression profiles
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通过 miRNA 和 mRNA 表达谱的综合分析确定 HepG2 细胞系的 RNA 表达特征
DOI：
10.1016/j.gene.2014.07.016
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发表时间：
2014-09-10
期刊：
影响因子：
3.5
通讯作者：
Lu, Zuhong
中科院分区：
文献类型：
--
作者：
Bai, Yunfei;Xue, Ying;Lu, Zuhong
了解miRNAs的调控网络和靶基因可以促进人类疾病（如癌症）治疗的发展。虽然许多有用的基因表达谱数据的肿瘤细胞系，微阵列数据的miRNA和mRNA的人HepG 2细胞系只与其他细胞系分别进行了比较。在HepG 2细胞的整合表达谱中miRNAs和mRNAs之间的关系仍然未知。为了探讨肝癌细胞中miRNA与mRNA的相关性，我们利用新一代测序技术在基因组水平上对HepG 2细胞和正常肝HL-7702细胞中的miRNA和mRNA表达进行了分析。我们鉴定了193个在这两种细胞系中差异表达的miRNAs。与HL-7702细胞相比，HepG 2细胞中有89个miRNAs表达下调，104个miRNAs表达上调。我们还观察到3035个mRNA在HepG 2细胞中显著失调。然后，我们对差异表达的miRNA和mRNA的表达数据进行了综合分析，发现了几个在HepG 2细胞中显著相关的miRNA-mRNA对。进一步的分析表明，这些差异表达的基因主要集中在ErbB、JAR-STAT、mTOR和WNT等4个肿瘤发生相关的信号通路中，而这些信号通路至今尚未得到充分的报道。本研究结果有助于进一步了解肝癌发生发展的机制。(C)2014爱思唯尔有限公司版权所有。
Understanding miRNAs' regulatory networks and target genes could facilitate the development of therapies for human diseases such as cancer. Although much useful gene expression profiling data for tumor cell lines is available, microarray data for miRNAs and mRNAs in the human HepG2 cell line have only been compared with that of other cell lines separately. The relationship between miRNAs and mRNAs in integrated expression profiles for HepG2 cells is still unknown. To explore the miRNA-mRNA correlations in hepatocellular carcinoma (HCC) cells, we performed miRNA and mRNA expression profiling in HepG2 cells and normal liver HL-7702 cells at the genome scale using next-generation sequencing technology. We identified 193 miRNAs that are differentially expressed in these two cell lines. Of these, 89 miRNAs were down-regulated in HepG2 cells compared with HL-7702 cells, while 104 miRNAs were up-regulated. We also observed 3035 mRNAs that are significantly dysregulated in HepG2 cells. We then performed an integrated analysis of the expression data for differentially expressed miRNAs and mRNAs and found several miRNA-mRNA pairs that are significantly correlated in HepG2 cells. Further analysis suggested that these differentially expressed genes were enriched in four tumorigenesis-related signaling pathways, namely, ErbB, JAR-STAT, mTOR, and WNT, which until now had not been fully reported. Our results could be helpful in understanding the mechanisms of HCC occurrence and development. (C) 2014 Elsevier B.V. All rights reserved.