Effect of titanium carbide coating on the osseointegration response in vitro and in vivo
Effect of titanium carbide coating on the osseointegration response in vitro and in vivo
复制标题
DOI：
10.1016/j.biomaterials.2006.08.018
复制
发表时间：
2007-02-01
期刊：
影响因子：
14
通讯作者：
Scandurra, Roberto
中科院分区：
文献类型：
--
作者：
Brama, Marina;Rhodes, Nicholas;Scandurra, Roberto
钛在牙科和骨科应用中的临床性能存在局限性。本研究描述了使用脉冲激光沉积 (PLD) 技术在钛基材上产生碳化钛 (TiC) 表面的涂层工艺，并评估了体外和体内的生物反应。 X射线光电子能谱（XPS）分析表明，表层存在18.6-21.5%的TiC，并伴有78.5-81.4%的钛氧化物，其浓度如下：11.1-13.0% Ti2O3、50.8-55.8% TiO2、14.5-14.7% TiO。通过半定量 PCR 测量，与未涂覆的钛相比，在 TiC 上生长的所有细胞系（原代人成骨细胞、hFOB1.19 和 ROS.MER#14）中成骨细胞分化的核心基因（碱性磷酸酶、A2 型 1 型胶原蛋白、骨钙素、BMP-4、TGF beta 和 Cbfa-1）的表达均上调 和实时 PCR，而参与破骨细胞生成和破骨细胞活性调节的基因（IL-6 和 M-CSF）则没有变化。与未涂层的钛相比，绵羊 2 周和 4 周以及兔子 4 周和 8 周后，TiC 涂层种植体周围的骨密度更高。仅两周后，通过活体染色观察，就在兔子模型中证明了快速骨沉积。结论是，与未涂层的钛相比，TiC 涂层可提高植入物硬度、通过表面稳定性提高生物相容性，并通过改善骨生长提高骨整合。 (c) 2006 Elsevier Ltd. 保留所有权利。
Titanium has limitations in its clinical performance in dental and orthopaedic applications. This study describes a coating process using pulsed laser deposition (PLD) technology to produce surfaces of titanium carbide (TiC) on titanium substrates and evaluates the biological response both in vitro and in vivo. X-ray photoelectron spectroscopy (XPS) analysis revealed the presence of 18.6-21.5% TiC in the surface layer, accompanied by oxides of titanium 78.5-81.4% in the following concentrations: 11.1-13.0% Ti2O3, 50.8-55.8% TiO2, 14.5-14.7% TiO. Expression of genes central to osteoblast differentiation (alkaline phosphatase, A2 pro-collagen type 1, osteocalcin, BMP-4, TGF beta and Cbfa-1) were up-regulated in all cell lines (primary human osteoblasts, hFOB1.19 and ROS.MER#14) grown on TiC compared with uncoated titanium when measured by semiquantitative PCR and real time-PCR, whilst genes involved in modulation of osteoclastogenesis and osteoclast activity (IL-6 and M-CSF) were unchanged. Bone density was shown to be greater around TiC-coated implants after 2 and 4 weeks in sheep and both 4 and 8 weeks in rabbits compared to uncoated titanium. Rapid bone deposition was demonstrated after only 2 weeks in the rabbit model when visualized with intravital staining. It is concluded that coating with TiC will, in comparison to uncoated titanium, improve implant hardness, biocompatibility through surface stability and osseointegration through improved bone growth. (c) 2006 Elsevier Ltd. All rights reserved.