Limitation of myocardial infarct size in the rabbit by ischaemic preconditioning is abolished by sodium 5-hydroxydecanoate.
Limitation of myocardial infarct size in the rabbit by ischaemic preconditioning is abolished by sodium 5-hydroxydecanoate.
复制标题
5-羟基癸酸钠消除了缺血预处理对家兔心肌梗塞面积的限制。
DOI：
10.1016/s0008-6363(96)00041-7
复制
发表时间：
1996
影响因子：
10.8
通讯作者：
C. Thiemermann
中科院分区：
文献类型：
--
作者：
E. Hide;C. Thiemermann
目的：探讨ATP敏感性钾通道（KATP）抑制剂5-羟基癸酸钠（5-HD）对戊巴比妥钠麻醉的新西兰白色白兔缺血预处理缩小心肌梗死范围的作用。缺血预处理是通过在60 min闭塞前先单次闭塞5 min LAL，然后再灌注15 min来实现的。在缺血预处理开始前10分钟，将缺血选择性KATP通道抑制剂5-HD（5 mg kg−1）推注至左心室。注射伊文思蓝染料，以确定在危险和梗死面积的左心室的面积由孵育的危险区与硝基蓝tetrazolium.Results：有没有组间的血液动力学或危险区的显着差异。缺血预处理组梗死面积（27 ± 8%）显著小于对照组（55 ± 3%）（P< 0.05）。5-HD预处理可完全阻断缺血预处理的心肌保护作用（50 ± 6%）.结论：5-HD预处理对心肌的保护作用依赖于ATP通道的开放。
Objective:To investigate the effect of the ATP-sensitive potassium (KATP) channel inhibitor sodium 5-hydroxydecanoate (5-HD) on the reduction in myocardial infarct size afforded by ischaemic preconditioning in the sodium pentobarbitone anaesthetised rabbit.Methods:New Zealand white rabbits were anaesthetised with sodium pentobarbitone and subjected to 60 min occlusion of the first antero-lateral branch of the left coronary artery (LAL) followed by 120 min reperfusion. Ischaemic preconditioning was achieved by a single episode of 5 min LAL occlusion followed by 15 min reperfusion prior to the 60 min occlusion. 5-HD (5 mg kg−1), an ischaemia selective KATPchannel inhibitor, was administered into the left ventricle as a bolus injection 10 min prior to the onset of ischaemic preconditioning. Injection of Evans blue dye was used to determine the area of the left ventricle at risk and infarct size was determined by incubation of the area at risk with nitro-blue tetrazolium.Results:There were no significant differences between groups in haemodynamics or area at risk. Ischaemic preconditioning resulted in a significant reduction in infarct size (27 ± 8%) when compared to control animals (55 ± 3%;P< 0.05). Pretreatment of animals with 5-HD completely abolished the cardioprotection seen with ischaemic preconditioning (50 ± 6%).Conclusion:These results support the hypothesis that the cardioprotection afforded by ischaemic preconditioning in the pentabarbitone anaesthetised rabbit is dependent on the opening of KATPchannels.