ESTIMATION AND MANIPULATION OF GLUTATHIONE LEVELS IN PREPUBERAL MOUSE OVARIES AND OVA - RELEVANCE TO SPERM NUCLEUS TRANSFORMATION IN THE FERTILIZED EGG
ESTIMATION AND MANIPULATION OF GLUTATHIONE LEVELS IN PREPUBERAL MOUSE OVARIES AND OVA - RELEVANCE TO SPERM NUCLEUS TRANSFORMATION IN THE FERTILIZED EGG
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DOI：
10.1002/mrd.1120140310
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发表时间：
1986-01-01
期刊：
影响因子：
--
通讯作者：
BLAKE, EJ
中科院分区：
文献类型：
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作者：
CALVIN, HI;GROSSHANS, K;BLAKE, EJ
Glutathione (GSH), the major low-molecular-weight thiol in mammalian cells, is believed to be a necessary factor for the transformation of the disulfide-stabilized sperms nucleus into the male pronucleus after fertilization. Its concentration in mouse ova, isolated from the ampulla of the oviduct after hormone-induced superovulation of 3-4-week-old mice, has been determined by an enzymic cycling microassay. The level found was 1.80 pmol per ovum. Mean ovum diameter was estimated as 71-72 .mu.m, indicating a GSH concentration of 9-10 mM in the mouse egg. Administration of L-buthionine S,R-sulfoximine, an inhibitor of GSH biosynthesis, during the 2 days preceding ovulation, reduced ovum GSH content below 0.20 pmol (< 1.0 mM). The mean GSH concentration of the hormone-stimulated ovaries was reduced from 3.2 mM to 0.2 mM under these conditions. It has also been demonstrated that measurement and manipulation of ovum and ovarian levels of GSH can aid in studying its function in ovaries, ova, and early embryos. Hormone-induced superovulation was achieved in BSO-treated prepuberal C57Bl/6 X SJL mice whose ovaries contained less than 10% of control levels of GSH. Over 50% of the isolated ova were fertilized in vitro. However, abnormal one-cell embryos resulted in which the maternally derived pronucleus coexisted with an unchanged sperm nucleus, thus confirming that adequate levels of GSH are necessary for initiating transformation of the fertilizing sperm nucleus.
