Expression profiling of intestinal tissues implicates tissue-specific genes and pathways essential for thyroid hormone-induced adult stem cell development.
Expression profiling of intestinal tissues implicates tissue-specific genes and pathways essential for thyroid hormone-induced adult stem cell development.
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DOI：
10.1210/en.2013-1432
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发表时间：
2013-08
期刊：
影响因子：
4.8
通讯作者：
Guihong Sun;R. Heimeier;L. Fu;Takashi Hasebe;Biswajit Das;A. Ishizuya‐Oka;Yunbo Shi
中科院分区：
文献类型：
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作者：
Guihong Sun;R. Heimeier;L. Fu;Takashi Hasebe;Biswajit Das;A. Ishizuya‐Oka;Yunbo Shi
脊椎动物肠道发育过程中上皮细胞的研究涉及生物学的许多当代方面：仅举几例，成体干细胞（ASC）的形成对于终身自我更新和干细胞活性的平衡至关重要。虽然已经对哺乳动物成体肠道干细胞的性质和功能进行了广泛的分析，但由于难以操纵晚期子宫封闭的胚胎，因此对其在发育过程中的形成知之甚少。两栖动物非洲爪蟾的胃肠道是研究哺乳动物ASC形成、细胞增殖和分化的极好模型系统。在T3依赖的两栖动物变态，消化道广泛重塑从幼虫到成人形式的两栖动物从其水生草食性的生活方式，陆地食肉青蛙的适应。这涉及ASC的从头形成，其需要在幼虫上皮和非上皮组织中的T3信号传导。为了了解潜在的分子机制，我们已经通过使用cDNA微阵列表征了上皮和非上皮组织中的基因表达谱。我们的研究结果表明，T3诱导不同的组织特异性基因调控程序与肠的重塑，特别是ASC的形成，并进一步表明存在潜在的许多新的干细胞相关基因，至少在肠道发育过程中。
The study of the epithelium during development in the vertebrate intestine touches upon many contemporary aspects of biology: to name a few, the formation of the adult stem cells (ASCs) essential for the life-long self-renewal and the balance of stem cell activity for renewal vs cancer development. Although extensive analyses have been carried out on the property and functions of the adult intestinal stem cells in mammals, little is known about their formation during development due to the difficulty of manipulating late-stage, uterus-enclosed embryos. The gastrointestinal tract of the amphibian Xenopus laevis is an excellent model system for the study of mammalian ASC formation, cell proliferation, and differentiation. During T3-dependent amphibian metamorphosis, the digestive tract is extensively remodeled from the larval to the adult form for the adaptation of the amphibian from its aquatic herbivorous lifestyle to that of a terrestrial carnivorous frog. This involves de novo formation of ASCs that requires T3 signaling in both the larval epithelium and nonepithelial tissues. To understand the underlying molecular mechanisms, we have characterized the gene expression profiles in the epithelium and nonepithelial tissues by using cDNA microarrays. Our results revealed that T3 induces distinct tissue-specific gene regulation programs associated with the remodeling of the intestine, particularly the formation of the ASCs, and further suggested the existence of potentially many novel stem cell-associated genes, at least in the intestine during development.