CLEAVAGE OF TRANSFER-RNA PRECURSORS BY THE RNA SUBUNIT OF ESCHERICHIA-COLI RIBONUCLEASE-P (M1 RNA) IS INFLUENCED BY 3'-PROXIMAL CCA IN THE SUBSTRATES
CLEAVAGE OF TRANSFER-RNA PRECURSORS BY THE RNA SUBUNIT OF ESCHERICHIA-COLI RIBONUCLEASE-P (M1 RNA) IS INFLUENCED BY 3'-PROXIMAL CCA IN THE SUBSTRATES
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DOI：
10.1016/0092-8674(84)90543-9
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
ALTMAN, S
中科院分区：
文献类型：
--
作者：
GUERRIERTAKADA, C;MCCLAIN, WH;ALTMAN, S
含有CCA序列的tRNA前体分子在所有成熟tRNA的3”末端发现，在体外比缺乏该序列的前体更容易被大肠杆菌RNase P的催化亚基M1 RNA切割。当前体被含有M1 RNA和蛋白亚基的重组RNase P全酶切割时，对CCA序列的敏感性不明显。这些结果是由大肠杆菌和人类染色体编码的单体前体分子和由噬菌体T4基因组编码的3个二聚体前体分子获得的。这些数据与之前关于T4 tRNA在体内生物合成的结果一致，表明CCA序列对tRNA前体的加工很重要。
tRNA precursor molecules that contain the CCA sequence found at the 3'' termini of all mature tRNA are cleaved in vitro more readily by M1 RNA, the catalytic subunit of E. coli RNase P, than precursors that lack this sequence. The sensitivity to the CCA sequence is not apparent when precursors are cleaved by the reconstituted RNase P holoenzyme that contains M1 RNA and the protein subunit. These results were obtained with monomeric precursor molecules encoded by the E. coli and human chromosomes and with 3 dimeric precursor molecules encoded by the phage T4 genome. The data are in agreement with previous results concerning T4 tRNA biosynthesis in vivo and show that the CCA sequence is important for the processing of precursors to tRNA.