Calcium binding proteins in motoneurons at low and high risk for degeneration in ALS
Calcium binding proteins in motoneurons at low and high risk for degeneration in ALS
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DOI：
10.1097/00001756-200010200-00009
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发表时间：
2000-10-20
期刊：
影响因子：
1.7
通讯作者：
Funk, GD
中科院分区：
文献类型：
--
作者：
Laslo, P;Lipski, J;Funk, GD
最近的报道挑战了钙结合蛋白的表达有助于肌萎缩性侧索硬化症（ALS）中一些运动神经元对变性的更大抵抗的假设。因此，我们使用免疫组织化学重新检测了calbindin， calretinin和parvalbumin在成年大鼠的易感（舌下，XII；和颈椎）和抵抗（动眼肌，III）运动神经元中的表达。运动核缺乏钙结合蛋白的免疫反应性，背角有较强的免疫反应性。Calretinin在脊髓运动神经元表达，III和XII运动神经元不表达。用多克隆抗体检测小白蛋白的免疫反应性，在脊髓和ill运动神经元中强烈，但在XII运动神经元中不强烈；单克隆抗体未见腹角染色。calretinin和parvalbumin在易感运动神经元中的差异表达表明，这些蛋白的免疫反应性并不是ALS抗变性的可靠标志。《神经科学报告》11:33 -33 (C) 2000。
Recent reports challenge the hypothesis that expression of calcium binding proteins contributes to the greater resistance of some motoneurons to degeneration in amyotrophic lateral sclerosis (ALS). We therefore re-examined, using immunohistochemistry, the expression of calbindin, calretinin and parvalbumin in vulnerable (hypoglossal, XII; and cervical spinal) and resistant (oculomotor, III) motoneurons of adult rats. Calbindin immunoreactivity was lacking in motor nuclei but strong in the dorsal horn. Calretinin was expressed in spinal, but not III or XII, motoneurons. Parvalbumin immunoreactivity, tested with a polyclonal antibody, was intense in spinal and ill, but not XII, motoneurons; however, no staining in the ventral horn was observed with a monoclonal antibody. Differential expression of calretinin and parvalbumin within vulnerable motoneurons suggests that immunoreactivity for these proteins is not a reliable marker for resistance to degeneration in ALS. NeuroReport 11:3305-3308 (C) 2000 Lippincott Williams & Wilkins.