Effect of RGD nanospacing on differentiation of stem cells.
Effect of RGD nanospacing on differentiation of stem cells.
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DOI：
10.1016/j.biomaterials.2013.01.021
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发表时间：
2013-04
期刊：
影响因子：
14
通讯作者：
Xuan Wang;Ce Yan;Kai Ye;Yao He;Zhenhua Li;Jiandong Ding
中科院分区：
文献类型：
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作者：
Xuan Wang;Ce Yan;Kai Ye;Yao He;Zhenhua Li;Jiandong Ding
制备了细胞粘附肽精氨酸-甘氨酸-天冬氨酸（RGD）在持续无污染聚（乙二醇）水凝胶上的纳米粒子，并在全血清水平下检查了间充质干细胞（MSC）在从37至124 nm的5个RGD纳米间距的图案上的行为8天。除细胞粘附外，在相应的培养基中观察到大鼠骨髓间充质干细胞的成骨和成脂诱导作用。我们不仅证实了先前在其他细胞类型（非干细胞）中报道的细胞扩散的纳米间距依赖性，例如在大于临界70 nm的纳米间距的情况下扩散较少，而且还发现了RGD纳米间距对干细胞谱系承诺的影响。成骨诱导和成脂诱导均导致大纳米间距的模式比小纳米间距的模式具有更高的分化程度。在混合成骨/成脂培养基中的共诱导下，在大的RGD纳米间距的模式上，成骨作用优于脂肪形成，尽管根据先前的研究，较少的细胞铺展本身对成骨作用不利，但对脂肪形成有利。RGD纳米间隔对干细胞谱系定型的影响是出乎意料的，并且不能通过细胞扩散效应来解释。因此，干细胞的分化可能固有地由材料表面上的生物活性配体的纳米间距调节。
Nanopatterns of a cell-adhesive peptide arginine–glycine–aspartate (RGD) on a persistently non-fouling poly(ethylene glycol) hydrogel were prepared, and behaviours of mesenchymal stem cells (MSCs) on patterns of five RGD nanospacings from 37 to 124 nm were examined under a full level of serum for eight days. Besides cell adhesion, osteogenic and adipogenic inductions of MSCs from rat bone marrow were observed in corresponding media. We not only confirmed the nanospacing dependence of cell spreading previously reported in other cell types (non-stem cells) such as less spreading in the case of nanospacings larger than the critical 70 nm, but also found the effect of RGD nanospacing on lineage commitments of stem cells. Both osteogenic and adipogenic inductions resulted in higher differentiation extents on patterns of large nanospacings than of small nanospacings. Under co-induction in the mixed osteogenic/adipogenic media, osteogenesis was predominant over adipogenesis on patterns of large RGD nanospacings, although a less cell spreading itself was beneficial not for osteogenesis but for adipogenesis according to previous studies without nanopatterns. The effect of RGD nanospacing on lineage commitments of stem cells is unexpected and cannot be interpreted via the cell spreading effect. Thus, the differentiation of stem cells might be regulated inherently by nanospacing of bioactive ligands on the material surfaces.