Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis
Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis
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用于高效在线净化拟除虫菊酯并随后进行高效液相色谱分析的类特异性免疫亲和整体材料
DOI：
10.1016/j.jchromb.2009.06.007
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发表时间：
2010-01-15
影响因子：
3
通讯作者：
Liu, Huwei
中科院分区：
文献类型：
--
作者：
Liang, Yuan;Zhou, Shuang;Liu, Huwei
建立了一种用于在线净化复杂样品中拟除虫菊酯类杀虫剂的整体免疫亲和色谱柱。用臭氧氧化溴氰菊酯生成(RS)-酰基-α-苯氧基苄基(RS)-顺式半抗原，将其与牛血清白蛋白偶联，制备了拟除虫菊酯类特异性抗体。聚甲基丙烯酸乙二醇酯-甲基丙烯酸缩水甘油酯在直径为50 mm、4.6 mm、带有两个辅助移液管头的不锈钢色谱柱中合成。实验证明，该聚合方法经济、重现性好。抗拟除虫菊酯抗体通过席夫碱反应与柱切换阀系统共价固定在整体载体上，免疫亲和性整体柱(IAM)可以很容易地适应反相高效液相色谱(HPLC)系统。在最佳的上柱、洗脱和洗脱条件下，IAM专属保留了溴氰菊酯、氟氰菊酯。采用C18色谱柱，以乙腈-水(83：17，V/V)为流动相，流速为1.0mL(min.建立的系统为高效在线净化各种样品中的拟除虫菊酯提供了一种高效的方法。(C)2009爱思唯尔B.V.保留所有权利。
A class-specific monolithic immunoaffinity column was developed for on-line clean-up of pyrethroid insecticides in complex samples Deltamethrin was oxidized with ozone to generate the hapten of (RS)-acyano-alpha-phenoxybenzyl (RS)-cis,traps-2,2-dimethyl-3-formyl-cyclopropane carboxylate Class-specific antibodies against pyrethroids were produced using the conjugate of above hapten with bovine serum albumin as the immunogen. Poly(ethylene dimethacrylate-glycidyl methacrylate) monolith was synthesized in a 50 mm, 4.6 mm id stainless steel cartridge with two auxiliary pipette tips. The polymerization method was proved to be economic and reproducible. Antibodies against pyrethrotds were covalently immobilized onto the monolithic support via Schiff base reaction With a column-switching valve system, the immunoaffinity monolith (IAM) could be readily adapted to the reversed-phase high-performance liquid chromatography (HPLC) system. Under the optimum loading, washing and eluting conditions, the IAM specifically retained deltamethrin, flumethrin. flucythrinate and cis/traps permethrin, which were further baseline separated by C18 column using acetonitrile-water (83:17, V/V) as the mobile phase at a flow rate of 1.0 mL(min. The established system provides a highly efficient approach for high-throughput on-line clean-up of pyrethroid in various samples. (C) 2009 Elsevier B.V. All rights reserved.