SIMULTANEOUS DETERMINATION OF MYOCARDIAL NUCLEOTIDES, NUCLEOSIDES, PURINE-BASES AND CREATINE-PHOSPHATE BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
SIMULTANEOUS DETERMINATION OF MYOCARDIAL NUCLEOTIDES, NUCLEOSIDES, PURINE-BASES AND CREATINE-PHOSPHATE BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/0378-4347(92)80222-c
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发表时间：
1992-07-01
期刊：
影响因子：
--
通讯作者：
HALLSTROM, S
中科院分区：
文献类型：
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作者：
FURST, W;HALLSTROM, S
本文介绍了一种离子对反相高效液相色谱法，用于心肌核苷酸、核苷及其代谢物和磷酸肌酸相关化合物的分离和定量。含有21种化合物的标准混合物的分离在5 μ m Hypersil ODS柱上进行，5分钟等度洗脱（缓冲液：0.1 M NaH2PO4，pH 5.5，含有5.9 mM四丁基硫酸氢铵），然后缓慢线性梯度至17%乙腈。该方法适用于提取物的冷冻大鼠心脏组织样品以及新生大鼠心脏心肌细胞的提取物，它提供了良好的分辨率的高能磷酸盐，包括磷酸肌酸，以及它们的降解产物。
An ion-pair reversed-phase high-performance liquid chromatographic method is described for the separation and quantification of myocardial nucleotides, nucleosides, their metabolites and creatine phosphate-related compounds in a single run. Separation of a standard mixture containing 21 compounds was achieved on a 5-mu-m Hypersil ODS column with a 5-min isocratic elution (buffer: 0.1 M NaH2PO4, pH 5.5, containing 5.9 mM tetrabutylammonium hydrogensulphate) followed by a slow linear gradient to 17% acetonitrile. The method was applied to extracts of freeze-clamped rat heart tissue samples as well as to extracts of neonatal rat heart cardiomyocytes, and it provided good resolution of high-energy phosphates, including creatine phosphate, as well as of their degradation products.