An analytical study of neocartilage from microtia and otoplasty surgical remnants: A possible application for BMP7 in microtia development and regeneration
An analytical study of neocartilage from microtia and otoplasty surgical remnants: A possible application for BMP7 in microtia development and regeneration
复制标题
DOI：
10.1371/journal.pone.0234650
复制
发表时间：
2020-06-17
期刊：
影响因子：
3.7
通讯作者：
Landis, William J.
中科院分区：
文献类型：
--
作者：
Childs, Robin DiFeo;Nakao, Hitomi;Landis, William J.
为了研究通过组织工程手段进行耳廓重建，本研究比较了从耳成形术后丢弃的小耳或正常（耳甲）组织中获得的人软骨细胞再生的软骨。将分离的细胞在体外扩增，接种到添加或不添加骨形态发生蛋白-7（BMP 7）的纳米聚乙醇酸（nanoPGA）片上，并植入裸鼠中10周。在标本收获时，通过大体形态学、组织学和RT-qPCR和微阵列分析评估软骨发育。正常和小耳畸形手术组织的新软骨被发现在其尺寸，蛋白多糖和弹性纤维染色的定性程度，以及II型和III型胶原蛋白，弹性蛋白和SOX 5的定量基因表达水平相等。微阵列分析，首次应用于正常和小耳新生软骨比较，没有产生在这两个样本组之间表达有统计学显著差异的基因。这些结果支持使用小耳畸形组织作为正常耳廓再造的细胞来源。正常和小耳畸形细胞的比较，每种细胞接种在nanoPGA上，并在缓释水凝胶中补充BMP 7，显示出通过微阵列分析鉴定的某些基因的统计学显著差异。在比较没有BMP 7的对应接种细胞的几项分析中也注意到这种差异。总结数据表明，BMP 7在小耳软骨再生中的可能应用，并鼓励进一步的研究，以阐明这种基因型差异是否转化为人类小耳畸形耳的表型特征。目前的工作进展的理解相关的潜在的临床应用的小耳外科手术残留物作为一个合适的细胞来源的组织工程的耳廓。
To investigate auricular reconstruction by tissue engineering means, this study compared cartilage regenerated from human chondrocytes obtained from either microtia or normal (conchal) tissues discarded from otoplasties. Isolated cells were expanded in vitro, seeded onto nanopolyglycolic acid (nanoPGA) sheets with or without addition of bone morphogenetic protein-7 (BMP7), and implanted in nude mice for 10 weeks. On specimen harvest, cartilage development was assessed by gross morphology, histology, and RT-qPCR and microarray analyses. Neocartilages from normal and microtia surgical tissues were found equivalent in their dimensions, qualitative degree of proteoglycan and elastic fiber staining, and quantitative gene expression levels of types II and III collagen, elastin, and SOX5. Microarray analysis, applied for the first time for normal and microtia neocartilage comparison, yielded no genes that were statistically significantly different in expression between these two sample groups. These results support use of microtia tissue as a cell source for normal auricular reconstruction. Comparison of normal and microtia cells, each seeded on nanoPGA and supplemented with BMP7 in a slow-release hydrogel, showed statistically significant differences in certain genes identified by microarray analysis. Such differences were also noted in several analyses comparing counterpart seeded cells without BMP7. Summary data suggest a possible application for BMP7 in microtia cartilage regeneration and encourage further studies to elucidate whether such genotypic differences translate to phenotypic characteristics of the human microtic ear. The present work advances understanding relevant to the potential clinical use of microtia surgical remnants as a suitable cell source for tissue engineering of the pinna.