Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution
Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution
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DOI：
10.1016/j.chom.2020.12.005
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发表时间：
2021-02-10
影响因子：
30.3
通讯作者：
Salehi-Ashtiani，Kourosh
中科院分区：
文献类型：
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作者：
Nelson，David R.;Hazzouri，Khaled M.;Salehi-Ashtiani，Kourosh
作为不同生态系统中不可或缺的初级生产者，微藻基因组可以用于生态学研究，但许多门缺乏具有代表性的基因组序列。我们对来自不同地理和气候的11个不同门的107种微藻进行了培养和测序。该集合用于解决咸水和淡水微藻之间的基因组差异。淡水物种在核和核膜功能上表现出以领域为中心的本体富集，而咸水物种在细胞器和细胞膜功能上表现出富集。此外，海洋物种的基因组中含有更多的病毒科（p = 8e-4）。氯病毒、球粒石病毒、潘多拉病毒、马赛病毒、图潘病毒和其他病毒的序列被发现整合到海洋环境藻类的基因组中。这些病毒起源序列被发现表达和编码多种功能。总之，本研究全面定义了微藻基因组中蛋白质编码和病毒元件的扩展范围，并提出了藻类耐盐性的统一适应策略。
Being integral primary producers in diverse ecosystems, microalgal genomes could be mined for ecological insights, but representative genome sequences are lacking for many phyla. We cultured and sequenced 107 microalgae species from 11 different phyla indigenous to varied geographies and climates. This collection was used to resolve genomic differences between saltwater and freshwater microalgae. Freshwater species showed domain-centric ontology enrichment for nuclear and nuclear membrane functions, while saltwater species were enriched in organellar and cellular membrane functions. Further, marine species contained significantly more viral families in their genomes (p = 8e–4). Sequences from Chlorovirus, Coccolithovirus, Pandoravirus, Marseillevirus, Tupanvirus, and other viruses were found integrated into the genomes of algal from marine environments. These viral-origin sequences were found to be expressed and code for a wide variety of functions. Together, this study comprehensively defines the expanse of protein-coding and viral elements in microalgal genomes and posits a unified adaptive strategy for algal halotolerance.