Telomere length variations in 6 mucosal cell types of gastric tissue observed using a novel quantitative fluorescence in situ hybridization method
Telomere length variations in 6 mucosal cell types of gastric tissue observed using a novel quantitative fluorescence in situ hybridization method
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DOI：
10.1016/j.humpath.2006.11.023
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发表时间：
2007-08-01
期刊：
影响因子：
3.3
通讯作者：
Takubo, Kaiyo
中科院分区：
文献类型：
--
作者：
Aida, Junko;Izumiyama-Shimomura, Naotaka;Takubo, Kaiyo
应用端粒和着丝粒肽核酸探针定量荧光原位杂交技术，对11例胃肿瘤组织中6种细胞类型的端粒长度进行了检测。我们的端粒长度估计值由背景校正的端粒强度除以背景校正的着丝粒强度（端粒与着丝粒比）确定。我们的研究结果表明，端粒长度在每个研究的情况下，依次减少成纤维细胞的胃底腺细胞，腺颈细胞，然后表面小窝细胞。然而，位于胃底腺中的结肠化细胞的端粒长度并不总是比其他细胞类型的端粒长度短，如先前其他人所报道的。幽门螺杆菌感染可引起胃底腺端粒缩短。虽然平均端粒长度在8例胃癌中各不相同，但端粒长度与Ki-67标记指数的相关性在与成纤维细胞测量标准化后建立。我们的结论是，我们的端粒与着丝粒比率方法可以可靠地估计胃粘膜中6种细胞类型的端粒长度，并阐明了癌细胞增殖活性与端粒长度之间的关系。(c)2007爱思唯尔公司All rights reserved.
We developed a novel method for evaluating telomere length in 6 cell types of noncancerous and cancerous mucosal tissues from 11 cases of gastric neoplasm using the quantitative fluorescence in situ hybridization method with telomere and centromere peptide nucleic acid probes. Our telomere length estimates were determined from the background-corrected telomere intensity divided by the background-corrected centromere intensity (telomere-to-centromere ratio). Our results indicated that telomere lengths in each of the cases studied were reduced in turn from fibroblasts to fundic gland cells, to glandular neck cells, and then to surface foveolar cells. However, the telomere lengths of intestinalized cells located among fundic glands were not always shorter than those of the other cell types, as reported previously by others. Helicobacter pylori infection was suggested to induce the telomere shortening seen in the fundic glands. Although the mean telomere lengths varied among the 8 gastric cancer cases, correlation of the telomere lengths with the Ki-67 labeling index was established after normalization with the fibroblast measurements. We conclude that our telomere-to-centromere ratio method can reliably estimate the telomere lengths of the 6 cell types in the gastric mucosa and clarifies the relationship between proliferative activity and the telomere length of cancer cells. (c) 2007 Elsevier Inc. All rights reserved.