Effects of Clarithromycin and Dexamethasone on Mucus Production in Isografted Rat Trachea
Effects of Clarithromycin and Dexamethasone on Mucus Production in Isografted Rat Trachea
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DOI：
10.1159/000322837
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发表时间：
2011-01-01
期刊：
影响因子：
3.1
通讯作者：
Majima, Yuichi
中科院分区：
文献类型：
--
作者：
Kitano, Masako;Ishinaga, Hajime;Majima, Yuichi
目的：本研究的目的是建立一种用于研究粘液过度产生的动物模型，并评估14元大环内酯类抗生素和糖皮质激素对脂多糖（LPS）诱导的粘液产生的影响。研究方法：将来自供体大鼠的气管同种移植到受体大鼠4周，并检查该气管同种移植模型在粘液产生研究中的有用性。结果如下：对受体大鼠口服克拉霉素（CAM）4周可显著降低LPS诱导的同种移植气管粘液生成。与对照组相比，地塞米松给药4周也显著减少了LPS处理的同种移植气管中的粘液体积。含有对照培养基的植入气管在组织学上与正常气管没有差异。当滴入植入气管的培养基含有1 μ g/ml LPS时，与滴入对照培养基的气管相比，气管腔中产生的粘液的体积和可旋转性显著增加。在含有LPS的植入气管中也观察到杯状细胞化生。结论：目前的研究表明，给予LPS的同种移植气管是上、下气道慢性高分泌性疾病的良好动物模型。CAM和地塞米松可作为此类高分泌性疾病的治疗选择。版权所有（C）2011 S. Karger AG，巴塞尔
Objectives:The purpose of this study was to develop an animal model for the study of mucus overproduction and to assess the effect of a 14-membered macrolide antibiotic and a glucocorticoid on lipopolysaccharide (LPS)-induced mucus production. Methods: Tracheas from donor rats were homografted to recipient rats for 4 weeks, and the usefulness of this tracheal homograft model in the study of mucus production was examined. Results: Oral administration of clarithromycin (CAM) to recipient rats for 4 weeks significantly reduced LPS-induced mucus production in the homografted trachea. Dexamethasone administered for 4 weeks also significantly reduced the mucus volume in LPS-treated homografted trachea compared with that in the control rats. The implanted trachea containing control medium was not histologically different from normal trachea. When the medium instilled into the implanted trachea contained 1 mu g/ml LPS, the volume and spinability of mucus produced in the tracheal lumen were significantly increased compared to those in the trachea instilled with control medium. Goblet cell metaplasia was also observed in the implanted trachea containing LPS. Conclusions: The present study shows that LPS-administered homografted trachea is a good animal model of chronic hypersecretory diseases of the upper and lower airways. CAM and dexamethasone could be treatment choices in such hypersecretory diseases. Copyright (C) 2011 S. Karger AG, Basel