Long-term video-EEG recordings following transient unilateral middle cerebral and common carotid artery occlusion in Long-Evans rats
Long-term video-EEG recordings following transient unilateral middle cerebral and common carotid artery occlusion in Long-Evans rats
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DOI：
10.1016/j.expneurol.2006.05.006
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发表时间：
2006-10-01
影响因子：
5.3
通讯作者：
Aronowski, Jaroslaw
中科院分区：
文献类型：
--
作者：
Kelly, Kevin M.;Jukkola, Peter I.;Aronowski, Jaroslaw
建立中风后癫痫发作和癫痫的脑损伤机制还不清楚，主要是因为这些现象的动物模型的发展有限。采用视频脑电图（video-electroencephalograms，EEG）技术，对2.5月龄雄性Long-Evans大鼠在假手术或短暂性单侧大脑中动脉（middle cerebral artery，MCA）和颈总动脉（common carotid artery，CCA）闭塞（MCA/CCAO）后6个月内的脑电行为特征进行了研究。本研究的主要结果是：（1）对照组动物在发作间期出现持续1- 2s的局灶性或局限性双侧7- 8Hz棘波放电（SWDs），而无行为学改变，发作期出现全身性7- 8Hz SWDs（失神发作），持续时间长，频率高，并与动物的运动停止有关;（2）损伤动物表现出与发作间期SWD类似的皮质梗死，除了局灶性放电相对于双侧放电更多，并且发作SWD，（3）与对照组相比，损伤组动物在7 Hz和15 Hz附近的半球和区域频谱功率降低，与发作期SWD的发生率降低直接相关;（4）病灶损伤不能独立地产生局灶性或全身性癫痫发作。这些研究表明，不同的Long-Evans大鼠大脑中动脉/CCAO损伤的青少年和监测视频脑电图记录在年轻的成年期，但未能提供中风后癫痫发作或癫痫的证据。(c)2006年爱思唯尔公司All rights reserved.
The mechanisms of injured brain that establish poststroke seizures and epilepsy are not well understood, largely because animal modeling of these phenomena has had limited development. We studied the electrobehavioral properties of 2.5-month-old mate Long-Evans rats by video-electroencephalogram (EEG) recordings during the 6 months following sham operation or lesioning by transient unilateral middle cerebral artery (MCA) and common carotid artery (CCA) occlusion (MCA/CCAO). The main findings of this study were: (1) control animals demonstrated interictal focal or restricted bilateral 7-8 Hz spike-wave discharges (SWDs) lasting 1-2 s without behavioral change and ictal generalized 7-8 Hz SWDs (absence seizures), which were prolonged, frequent, and associated with motor arrest of the animal; (2) lesioned animals demonstrated cortical infarction associated with interictal SWDs similar to controls, except that focal discharges were more numerous relative to bilateral discharges, and ictal SWDs, which were of shorter duration and less frequent than those of controls; (3) lesioned animals demonstrated decreased hemispheric and regional spectral power at similar to 7 and 15 Hz compared with controls, directly related to the reduced occurrence of ictal SWDs; and (4) lesioning did not independently generate either focal or generalized epileptic seizures. These studies demonstrate distinct electrobehavioral properties of Long-Evans rats lesioned by MCA/CCAO as juveniles and monitored by video-EEG recordings during young adulthood but fail to provide evidence of poststroke seizures or epilepsy. (c) 2006 Elsevier Inc. All rights reserved.